Institute for Biochemistry and Molecular Biology, Department of Chemistry, Faculty of Mathematics, Computer Science and Natural Science, Hamburg University, Hamburg, Germany.
Institute for Microbiology, Faculty for Mathematics and Natural Science, University Kiel, Kiel, Germany.
Methods Mol Biol. 2024;2741:175-181. doi: 10.1007/978-1-0716-3565-0_9.
RNA double-strand hybridization is a key player in gene expression regulation. Single-stranded RNA of up to 300 nucleotides forms Watson-Crick base pairs with complementary messenger RNA. Fluorescence-based single-molecule methods allow to study RNA-RNA interaction under physiological conditions. Here is described, how the dissociation constant of RNA double strands can be determined by applying fluorescence correlation spectroscopy.
RNA 双链杂交是基因表达调控的关键因素。多达 300 个核苷酸的单链 RNA 与互补的信使 RNA 形成 Watson-Crick 碱基对。基于荧光的单分子方法允许在生理条件下研究 RNA-RNA 相互作用。本文描述了如何通过应用荧光相关光谱法来确定 RNA 双链的离解常数。
