溴代靛玉红 FLIM/PLIM 传感器用于测量常氧和低氧 PDT 条件下的氧含量。
Bromine indirubin FLIM/PLIM sensors to measure oxygen in normoxic and hypoxic PDT conditions.
机构信息
University Ulm, Core Facility Confocal and Multiphoton Microscopy N24, Albert-Einstein-Allee 11, 89081 Ulm, Germany; University of Coimbra, CQC-ISM, Department of Chemistry, Coimbra, P3004-535, Portugal.
University Ulm, Core Facility Confocal and Multiphoton Microscopy N24, Albert-Einstein-Allee 11, 89081 Ulm, Germany.
出版信息
Photodiagnosis Photodyn Ther. 2024 Feb;45:103964. doi: 10.1016/j.pdpdt.2024.103964. Epub 2024 Jan 11.
BACKGROUND
The induction of phototoxicity during photodynamic therapy (PDT) is dependent on oxygen availability. For this reason, the development of sensors to measure oxygen and oxygen consumption is extremely important.
APPROACH
In this project we have used Fluorescence Lifetime imaging (FLIM) and Phosphorescence Lifetime Imaging/ delayed Fluorescence Lifetime Imaging (PLIM/dFLIM) to investigate the ability of bromine indirubin derivatives as oxygen sensors.
RESULTS
The oxygen sensitivity of bromine indirubins was detected through PLIM/dFLIM. Moreover, we have observed, by measuring nicotinamide adenine dinucleotide (NADH) FLIM, that bromine indirubin has a significant impact on cellular metabolism by shifting the SCC-4 Cells metabolism from oxidative phosphorylation (OXPHOS) to glycolysis.
CONCLUSIONS
In conclusion, this study successfully achieves its goals and provides important insights into the use of indirubin as a potential oxygen consumption sensor with the capability to identify and differentiate between normoxic and hypoxic regions within the cells.
背景
光动力疗法（PDT）过程中光毒性的诱导取决于氧气的可用性。出于这个原因，开发用于测量氧气和耗氧量的传感器非常重要。
方法
在这个项目中，我们使用荧光寿命成像（FLIM）和磷光寿命成像/延迟荧光寿命成像（PLIM/dFLIM）来研究溴靛红衍生物作为氧传感器的能力。
结果
通过 PLIM/dFLIM 检测到溴靛红的氧敏感性。此外，通过测量烟酰胺腺嘌呤二核苷酸（NADH）FLIM，我们观察到溴靛红对细胞代谢有显著影响，将 SCC-4 细胞的代谢从氧化磷酸化（OXPHOS）转变为糖酵解。
结论
总之，本研究成功达到了其目标，并为靛红作为一种潜在的氧耗传感器的使用提供了重要的见解，该传感器具有识别和区分细胞内正常氧和缺氧区域的能力。
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