水凝胶透明质酸对人牙髓干细胞牙本质涎磷蛋白表达的影响。

The Effect of Hydrogel Hyaluronic Acid on Dentine Sialophosphoprotein Expression of Human Dental Pulp Stem Cells.

机构信息

Department of Conservative Dentistry, Universitas Indonesia Faculty of Dentistry, Jakarta, Indonesia.

Department of Dermatology and Venereology, Universitas Sebelas Maret Faculty of Medicine, Surakarta, Indonesia.

出版信息

Eur Endod J. 2023 Aug;8(4):280-285. doi: 10.14744/eej.2023.59672.

DOI:10.14744/eej.2023.59672

PMID:38219035

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10500214/

Abstract

OBJECTIVE

Hyaluronic acid (HA) is glycosaminoglycan and one of important factors in extracellular matrix. In an inflamed pulp, when niche biology is conducive, the recruitment of human dental pulp stem cells (hDPSCs) will take place and differentiate into odontoblast like cell, creating reparative dentine and expressing dentine sialophosphoprotein (DSPP). Therefore, the purpose of this study was to analyze the potential of hydrogel HA in various concentration towards hDPSCs differentiation via DSPP expression at day 7 and 14.

METHODS

After hDPSCs incubation reaching 80% confluence, cells were then starved for 24 hours. Then, culture media were supplemented with osteogenic media. hDPSCs planted into 96 well plate and HA 10 μg/mL, 20 μg/mL, and 30 μg/mL were added. DSPP expression was analysed using elisa reader at day 7 and 14, qualitative result was analysed using alizarin red at day 21. Data was analysed using one-way ANOVA.

RESULTS

At day 7, there was a statistically significant different potential of HA conditioned media in various concentration (p<0.05) towards hDPSCs differentiation via expression of DSPP with HA 30 μg/mL being the most potential concentration to increase DSPP expression.

CONCLUSION

HA have the potential to increase odontoblast differentiation process via expression of DSPP, with HA 30 μg/mL being the optimum concentration for hDPSCs. (EEJ-2022-12-169).

摘要

目的

透明质酸（HA）是糖胺聚糖的一种，也是细胞外基质的重要因素之一。在发炎的牙髓中，当龛位生物学有利于其发展时，人牙髓干细胞（hDPSCs）将被招募并分化为成牙本质样细胞，形成修复性牙本质并表达牙本质涎磷蛋白（DSPP）。因此，本研究旨在通过分析不同浓度的水凝胶 HA 在第 7 天和第 14 天通过 DSPP 表达对 hDPSCs 分化的潜在作用。

方法

当 hDPSCs 达到 80%汇合度时，将其孵育 24 小时。然后，用成骨培养基饥饿细胞。将 hDPSCs 种植到 96 孔板中，并加入 10μg/mL、20μg/mL 和 30μg/mL 的 HA。使用 ELISA 读取器在第 7 天和第 14 天分析 DSPP 的表达，在第 21 天使用茜素红进行定性分析。使用单因素方差分析对数据进行分析。