Laboratory of Inflammation and Translational Gastroenterology (LIGAT), Postgraduate Program in Biotechnology (PPGBIOTEC), Federal University of Delta do Parnaíba, Av. São Sebastião, 2819, Parnaíba, PI CEP 64202-020, Brazil.
Center for Research in Applied Morphology and Immunology, NuPMIA, University of Brasília, Brasília, Brazil; Research Center on Biodiversity and Biotechnology (BIOTEC), Federal University of Delta do Parnaíba, UFDPar, Parnaíba, PI CEP 64202-020, Brazil.
Int J Biol Macromol. 2024 Mar;260(Pt 1):129397. doi: 10.1016/j.ijbiomac.2024.129397. Epub 2024 Jan 14.
Biotechnological advancements require the physicochemical alteration of molecules to enhance their biological efficacy for the effective treatment of gastric ulcers. The study aimed to produce a polyelectrolytic compound from red angico gum (AG) by carboxymethylation, evaluate its physicochemical characteristics and investigate gastric protection against ethanol-induced ulcers. AG and carboxymethylated angico gum (CAG) were characterized by Fourier transform infrared spectroscopy, determination of the degree of substitution and gel permeation chromatography (GPC) and C NMR techniques. The results demonstrated that the modification of the polymer was satisfactory, presenting conformational changes e improving the interaction with the gastric mucosa. AG and CAG reduced macroscopic and microscopic damage such as edema, hemorrhage and cell loss caused by exposure of the mucosa to alcohol. Both demonstrated antioxidant activity in vitro, and in vivo, pretreatment with gums led to the restoration of superoxide dismutase and glutathione levels compared to the injured group. Concurrently, the levels of malondialdehyde and nitrite decreased. Atomic force microscopy showed that CAG presented better conformational properties of affinity and protection with the gastric mucosa compared to AG in the acidic pH. Based on our findings, it is suggested that this compound holds promise as a prospective product for future biotechnological applications.
生物技术的进步需要对分子进行物理化学修饰，以提高其生物功效，从而有效治疗胃溃疡。本研究旨在通过羧甲基化从红檀树胶（AG）中制备一种聚电解质化合物，评估其物理化学特性，并研究其对乙醇诱导的溃疡的胃保护作用。AG 和羧甲基化的红檀树胶（CAG）通过傅里叶变换红外光谱、取代度的测定和凝胶渗透色谱（GPC）和 C NMR 技术进行了表征。结果表明，聚合物的修饰是令人满意的，呈现出构象变化，提高了与胃黏膜的相互作用。AG 和 CAG 减少了暴露于酒精的黏膜的宏观和微观损伤，如水肿、出血和细胞损失。两者均表现出体外抗氧化活性，与损伤组相比，用胶预处理可使超氧化物歧化酶和谷胱甘肽水平恢复。同时，丙二醛和亚硝酸盐的水平降低。原子力显微镜显示，与 AG 相比，CAG 在酸性 pH 下具有更好的亲和性和保护胃黏膜的构象特性。基于我们的发现，建议该化合物作为未来生物技术应用的有前途的产品。
