Jiangsu Key Laboratory of Oral Diseases, Jiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing Medical University, Nanjing 210029, PR China; Jiangsu Collaborative Innovation Center for Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, PR China.
Jiangsu Collaborative Innovation Center for Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210023, PR China.
Int J Biol Macromol. 2024 Feb;259(Pt 2):129402. doi: 10.1016/j.ijbiomac.2024.129402. Epub 2024 Jan 12.
Getting rid of the biofilms is a major challenge when treating skin and soft tissue infections (SSTI), an inflammatory illness brought on by bacteria. Traditional magnetic materials have a limited dispersibility and a biofilm permeable property, making it challenging to remove biofilms and causing infection to linger. To solve these problems, we developed a kind of magnetic composite nanoplatform coated with indocyanine green carbon dots and modified with chitosan modification (Fe-ICGCDs@CS). Fe-ICGCDs@CS has high dispersibility and improves the conductivity of biofilms under magnetic action. Fe-ICGCDs@CS can adsorb bacteria via the positive charge and achieve precise photothermal sterilization and photodynamic therapy (PDT). Moreover, by catalyzing hydrogen peroxide (2 mM), Fe-ICGCDs@CS can produce oxygen to relieve the anoxic state in the deep layer of biofilms and activate dormant bacteria to make them sensitive to external stimuli. All in all, unlike the common "just kill" sterilization model, Fe-ICGCDs@CS can accurately kill bacteria and be recovered by an external magnetic field at the end of treatment, thus reducing the potential biological toxicity of nanomaterials. Therefore, the proposed Fe-ICGCDs@CS provides a new antibacterial method with low biotoxicity for clinical application in the treatment of biofilm infections.
在治疗皮肤和软组织感染（SSTI）这种由细菌引起的炎症性疾病时，摆脱生物膜是一个主要挑战。传统的磁性材料分散性有限，且具有生物膜渗透性，难以去除生物膜，导致感染持续存在。为了解决这些问题，我们开发了一种涂有吲哚菁绿碳点并经壳聚糖修饰的磁性复合纳米平台（Fe-ICGCDs@CS）。Fe-ICGCDs@CS 具有高分散性，并在磁场作用下提高生物膜的导电性。Fe-ICGCDs@CS 可以通过正电荷吸附细菌，实现精确的光热杀菌和光动力疗法（PDT）。此外，通过催化 2mM 的过氧化氢，Fe-ICGCDs@CS 可以产生氧气，缓解生物膜深层的缺氧状态，并激活休眠细菌，使它们对外界刺激敏感。总之，与常见的“仅仅杀死”杀菌模式不同，Fe-ICGCDs@CS 可以在治疗结束时通过外部磁场准确杀死细菌并回收，从而降低纳米材料的潜在生物毒性。因此，所提出的 Fe-ICGCDs@CS 为临床应用于治疗生物膜感染提供了一种低生物毒性的新的抗菌方法。
