Yan Chaoren, Shao Xu, Shu Qi, Teng Yonggang, Qiao Youbei, Guan Ping, Hu Xiaoling, Wang Chaoli
Department of Chemistry, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, Xi'an 710072, PR China.
Department of Chemistry, School of Chemistry and Chemical Engineering, Northwestern Polytechnical University, Xi'an 710072, PR China.
Int J Biol Macromol. 2021 Sep 1;186:839-848. doi: 10.1016/j.ijbiomac.2021.07.078. Epub 2021 Jul 17.
Antibacterial photodynamic therapy (PDT) has attracted extremely attention due to not inducing bacteria to generate resistance. However, the poor utilization and low reactive oxygen species (ROS) field of photosensitizers hinder their further application for antibacterial. Here, we designed ultra-thin hollow silica nanoparticles (UHSN), followed by pore-engineering including covalent anchoring of chitosan (UHSN@CS) for enhanced loading and photodynamic property of photosensitizer. The UHSN@CS exhibit high loading efficiency (80.6%, pH = 6.0) and controllable pH-responsive release for Ce6. Additionally, UHSN@CS can enhance the ROS yield of photosensitizers and effectively adhere to S. aureus, thus enormously enhancing antibacterial performance toward bacteria. Moreover, UHSN@CS-Ce6 can obliterate mature S. aureus biofilm and cause an 81% decrease in the biomass, showing a better therapeutic effect than Ce6 (59.2%) under laser irradiation. In vivo results confirm that UHSN@CS-Ce6 is effective to promote infectious wound regeneration. As photodynamic-based nanoplatforms, UHSN@CS-Ce6 are potential antibacterial agents for skin infection therapy.
抗菌光动力疗法（PDT）因其不会诱导细菌产生耐药性而备受关注。然而，光敏剂的低利用率和低活性氧（ROS）产率阻碍了它们在抗菌方面的进一步应用。在此，我们设计了超薄中空二氧化硅纳米颗粒（UHSN），随后进行孔工程，包括壳聚糖的共价锚定（UHSN@CS），以提高光敏剂的负载量和光动力性能。UHSN@CS对Ce6表现出高负载效率（80.6%，pH = 6.0）和可控的pH响应释放。此外，UHSN@CS可以提高光敏剂的ROS产率，并有效粘附于金黄色葡萄球菌，从而极大地增强对细菌的抗菌性能。此外，UHSN@CS-Ce6可以消除成熟的金黄色葡萄球菌生物膜，并使生物量减少81%，在激光照射下显示出比Ce6（59.2%）更好的治疗效果。体内结果证实，UHSN@CS-Ce6对促进感染伤口再生有效。作为基于光动力的纳米平台，UHSN@CS-Ce6是用于皮肤感染治疗的潜在抗菌剂。
