Habib Zakia, Ijaz Siddra, Haq Imran Ul
Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, University Road, Faisalabad, Pakistan.
Department of Plant Pathology, University of Agriculture, University Road, Faisalabad, Pakistan.
Physiol Mol Biol Plants. 2023 Dec;29(12):1959-1979. doi: 10.1007/s12298-023-01398-2. Epub 2023 Dec 8.
possesses remarkable nutritional value and adaptability to various agroecological conditions. Panicle architecture influences the number of spikelets and grains in a panicle, ultimately leading to productivity and yield. Therefore, this study aimed to investigate the metabolites, nutrients, and minerals in accessions of varying panicle architecture. Metabolic profiling using liquid chromatography-mass spectrometry (LC-MS) analysis identified seventeen metabolites, including flavonoids, phenolics, fatty acids, terpenoids, phenylbutenoid dimers, amino acids, and saccharides. Eight metabolic compounds were reported in this study for the first time in quinoa. Some metabolites were detected as differentially expressed. The compound (Z)-1-(2,4,5-trimethoxyphenyl) butadiene and chrysin were found only in SPrecm. Sodium ((2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxtetrahydrofuran-2-yl) methyl hydrogen phosphate and elenolic acid were detected only in CHEN-33, and quercetin, 3-hydroxyphloretin-3'-C-glucoside, kurarinone, and rosmarinic acid were identified only in D-12175. Variable importance in projection (VIP) scores annotated ten metabolites contributing to variability. Mineral analysis using atomic absorption spectrophotometry indicated that the quantity of magnesium and calcium is high in D-12175. In comparison, SPrecm showed a high quantity of magnesium compared to CHEN-33, while CHEN-33 showed a high quantity of calcium compared to SPrecm. However, the proximate composition showed no significant difference among quinoa accessions.
The online version contains supplementary material available at 10.1007/s12298-023-01398-2.
具有显著的营养价值，并能适应各种农业生态条件。穗型结构影响穗中小穗和籽粒的数量，最终影响生产力和产量。因此，本研究旨在调查不同穗型结构的藜麦品种中的代谢产物、营养成分和矿物质。使用液相色谱 - 质谱联用（LC - MS）分析进行代谢谱分析，鉴定出17种代谢产物，包括黄酮类、酚类、脂肪酸、萜类、苯基丁烯类二聚体、氨基酸和糖类。本研究首次在藜麦中报道了8种代谢化合物。检测到一些代谢产物存在差异表达。化合物（Z）-1-（2,4,5-三甲氧基苯基）丁二烯和白杨素仅在SPrec m中发现。磷酸氢钠（（2R,3S,4R,5R）-5-（6-氨基-9H-嘌呤-9-基）-3,4-二羟基四氢呋喃-2-基）甲基酯和橄榄酸仅在CHEN - 33中检测到，而槲皮素、3-羟基根皮苷-3'-C-葡萄糖苷、苦参酮和迷迭香酸仅在D - 12175中鉴定到。投影变量重要性（VIP）评分标注出10种导致变异的代谢产物。使用原子吸收分光光度法进行的矿物质分析表明，D - 12175中镁和钙的含量较高。相比之下，与CHEN - 33相比，SPrec m中镁含量较高，而与SPrec m相比，CHEN - 33中钙含量较高。然而，藜麦品种之间的近似成分没有显著差异。
在线版本包含可在10.1007/s12298-023-01398-2获取的补充材料。
