采用高效液相色谱-质谱联用仪测定苹果渣（金冠品种）的多酚谱
Determination of Polyphenolic Profile of Apple Pomace ( Golden Delicious Variety) by HPLC-MS.
作者信息
Orozco-Flores Laura A, Salas Erika, Rocha-Gutiérrez Beatriz, Peralta-Pérez María Del R, González-Sánchez Guillermo, Ballinas-Casarrubias Lourdes
机构信息
Facultad de Ciencias Químicas, Universidad Autónoma de Chihuahua (UACH), Circuito Universitario Campus II, Chihuahua, Chihuahua 31125, Mexico.
Departamento de Medio Ambiente y Energía, Centro de Investigación en Materiales Avanzados (CIMAV), Miguel de Cervantes 120 Complejo Industrial Chihuahua, Chihuahua, Chihuahua 31136, Mexico.
出版信息
ACS Omega. 2023 Dec 15;9(1):196-203. doi: 10.1021/acsomega.3c03218. eCollection 2024 Jan 9.
Apple ( Borkh) is an appreciated source of polyphenols. Phenolic compounds are known as natural antioxidants and have a wide range of applications in different industries. Apple pomace has the potential of being an alternative source of polyphenols. To determine the polyphenolic profile of apple pomace, samples from the skin at two different stages of ripening were extracted with 80-20% EtOH-water/acetic acid 5% (S1) and 20-80% EtOH-water/acetic acid 5% (S2) in order to determine the solvent system. Ripe skins extracted with S1 showed a higher total polyphenol content or TPC (1.21 g of polyphenols per 100 g of fresh weight (FW)) than unripe apple skin, being the most effective system tested and a mean degree of polymerization of 2.47. Commercial apple pomace was extracted with S1, resulting in a TPC of 0.5615 ± 0.007 g of polyphenols per 100 g of FW. Meanwhile, the RP-HPLC-MS analysis led to the tentative identification of several polyphenolic compounds.
苹果（博尔克品种）是一种备受青睐的多酚来源。酚类化合物被认为是天然抗氧化剂，在不同行业有着广泛应用。苹果渣有潜力成为多酚的替代来源。为了确定苹果渣的多酚谱，从两个不同成熟阶段的果皮中取样，用80 - 20%乙醇 - 水/5%乙酸（S1）和20 - 80%乙醇 - 水/5%乙酸（S2）进行提取，以确定溶剂系统。用S1提取的成熟果皮显示出比未成熟苹果皮更高的总多酚含量（TPC）（每100克鲜重（FW）含1.21克多酚），是测试的最有效系统，平均聚合度为2.47。用S1提取商业苹果渣，得到的TPC为每100克FW含0.5615±0.007克多酚。同时，反相高效液相色谱 - 质谱分析初步鉴定出了几种多酚化合物。
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