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苹果（Borkh）中多酚类化合物的定量分析以及苯丙烷类途径响应感染的相关基因表达研究。

Quantification of polyphenolic compounds and relative gene expression studies of phenylpropanoid pathway in apple ( Borkh) in response to infection.

作者信息

Mansoor Sheikh, Sharma Vikas, Mir Mudasir A, Mir Javid I, Un Nabi Sajad, Ahmed Nazeer, Alkahtani Jawaher, Alwahibi Mona S, Masoodi Khalid Z

机构信息

Division of Biochemistry, Faculty of Basic Sciences, SKUAST, Jammu 180009, India.

Division of Plant Biotechnology, SKUAST, Shalimar, Kashmir 190025, India.

出版信息

Saudi J Biol Sci. 2020 Dec;27(12):3397-3404. doi: 10.1016/j.sjbs.2020.09.007. Epub 2020 Sep 10.

DOI:10.1016/j.sjbs.2020.09.007
PMID:33304148
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7715465/
Abstract

Apple ( Borkh) is rich in phenolic compounds, which may enhance resistance to scab disease caused by . In present study, apple cv. Golden Delicious was used for estimation of total phenols, flavonoids and quantification of six individual phenolic compounds. between control vs inoculated samples at different inoculation stages. The relative gene expression of phenylalanine ammonia lyase, chalcone synthase and flavanone 3 hydroxylase increased and polyphenolic compounds were constitutively upregulated at different post-inoculation stages. Data suggest that synthesis and accumulation of polyphenols is closely related with disease resistance against This study may play a vital role in understanding and finding out the governing mechanisms of scab resistance.

摘要

苹果（博尔克）富含酚类化合物，这可能增强对由[病原菌名称缺失]引起的疮痂病的抗性。在本研究中，苹果品种金冠用于总酚、类黄酮的测定以及六种单一酚类化合物的定量分析。比较了不同接种阶段对照样品与接种样品之间的情况。苯丙氨酸解氨酶、查尔酮合酶和黄烷酮3 -羟化酶的相对基因表达增加，并且在不同接种后阶段多酚类化合物持续上调。数据表明，多酚的合成与积累与对[病原菌名称缺失]的抗病性密切相关。本研究可能在理解和找出抗疮痂病的调控机制方面发挥重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee7c/7715465/0ce8518c482f/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee7c/7715465/05c095400b90/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee7c/7715465/7efc4227af69/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee7c/7715465/f053314ece73/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee7c/7715465/73655e47e3c8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee7c/7715465/8572babdb405/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee7c/7715465/0ce8518c482f/gr6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee7c/7715465/05c095400b90/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee7c/7715465/7efc4227af69/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee7c/7715465/f053314ece73/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee7c/7715465/73655e47e3c8/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee7c/7715465/8572babdb405/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ee7c/7715465/0ce8518c482f/gr6.jpg

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