Validation of a leg-mounted pedometer for the measurement of steps in lactating Holstein cows.

The aim of this study was to validate the pedometer AfiAct II (AfiMilk) for the measurement of steps in lactating Holstein cows housed in a freestall design by assessing its agreement with visual observation of step counts. A total of 41 primiparous (n = 12) and multiparous (n = 29) cows were enrolled in the study between August and September 2018. Steps were monitored continuously by the pedometer and visually assessed for a 24-h period using video recordings. Visually observed steps were categorized as walking and stationary steps. The total number of steps taken per cow was calculated using the sum of walking and stationary steps. Unprocessed step count data from the study day were retrieved from the AfiMilk system in time-blocks of approximately 15 min. Repeated measures correlation was used to quantify the association between the pedometer measurements and visual observation of step counts. Nonindependence among observations were accounted adjusting for interindividual (cow) variability with an analysis of covariance. Pearson correlation coefficients (r) were categorized from negligible (0.00-0.30) to very high (0.90-1.00). Bland-Altman plots were created to evaluate the bias between the pedometer and visual observations. A total of 2,261 time-blocks were used in this study with an average (mean ± standard deviation) of 55.14 ± 8.1 time-blocks per cow. A high correlation was found for the evaluation between the pedometer and observed walking steps (r = 0.74; 95% confidence interval [CI] = 0.73-0.76), stationary steps (r = 0.71; 95% CI = 0.69-0.63), and total steps (r = 0.88; 95% CI = 0.87-0.89). The results of the Bland-Altman plot suggested limited bias between the pedometer step counts and visual observation of steps, independent of the type of steps. Numerical differences and several time-block differences outside of the 95% interval of agreement suggested an overestimation of step counts by the pedometer, which increased as the number of steps increased. The pedometer measured, on average, 97.6 ± 118.5 (28%), 249.2 ± 126.2 (125%), and 297.2 ± 205.4 (196%) steps/day more than the visual observed total steps, stationary steps, and walking steps, respectively. Our findings indicate that the pedometer counts all movement in which the pedometer leg is lifted off the floor without distinguishing if there was body movement of the animal.