草药 Emelia-M、Mshikazi 和 Delosma H 对人白血病细胞的抗癌作用。
Anticancer effects of herbal medicine Emelia-M, Mshikazi and Delosma H against human leukaemia cells.
机构信息
Traditional Medicine Laboratory, School of Nursing and Public Health, College of Health Sciences, University of KwaZulu-Natal.
Department of Pharmacy, School of Health care Sciences, University of Limpopo.
出版信息
Afr Health Sci. 2023 Jun;23(2):305-329. doi: 10.4314/ahs.v23i2.35.
BACKGROUND
Leukaemia is one of the three major types of blood cancers that lead to the overproduction of abnormal white blood cells. (EMB), and are herbal medicines that are being used by traditional healers in KwaZulu-Natal, South Africa to treat leukaemia and other diseases.
OBJECTIVES
To gain insight into the safety (non-toxic effect), anti-cancer activity, mechanisms of action and phytochemical profiles of traditional herbal medicines and in South Africa.
METHODS
The viability of human peripheral blood mononuclear cells (PBMCs), monocytic (THP-1) and T-lymphocyte (Jurkat) cell lines exposed to varying concentrations of aqueous extracts of the three herbal medicines were assessed using adenosine triphosphate (ATP) assay.
RESULTS
All three extracts showed a dose-dependent effect on the viability of PBMCs. Cell viability decreased with increasing concentrations of extracts when compared with the untreated cells at 24 and 48 hours. The inhibitory activities (IC50) of the extract were found in the order of . All the extracts induced apoptosis with minimal necrosis. Many bioactive compounds that have been previously reported to have anticancer effects were identified in the extracts.
CONCLUSION
extract significantly inhibited the growth of THP-1 and Jurkat cells and induced cell death through apoptosis than the other two extracts.
背景
白血病是三种主要血液癌之一，导致异常白细胞过度产生。（EMB）和 是南非夸祖鲁-纳塔尔省的传统治疗师用来治疗白血病和其他疾病的草药。
目的
深入了解南非传统草药 和 的安全性（无毒作用）、抗癌活性、作用机制和植物化学成分。
方法
使用三磷酸腺苷 (ATP) 测定法评估人类外周血单核细胞 (PBMC)、单核细胞 (THP-1) 和 T 淋巴细胞 (Jurkat) 细胞系暴露于三种草药的水提物的不同浓度时的活力。
结果
三种提取物均表现出对 PBMC 活力的剂量依赖性影响。与未经处理的细胞相比，在 24 和 48 小时时，细胞活力随着提取物浓度的增加而降低。提取物的抑制活性 (IC50) 按 的顺序排列。所有提取物均诱导凋亡，而坏死最小。在提取物中鉴定出许多先前报道具有抗癌作用的生物活性化合物。
结论
与其他两种提取物相比， 提取物可显著抑制 THP-1 和 Jurkat 细胞的生长，并通过凋亡诱导细胞死亡。
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