Hemoglobin plays a vital role in a series of biological activities. Abnormal levels of hemoglobin in blood are associated with many clinical diseases. Therefore, development of simple and accurate methods for sensing hemoglobin is of considerable significance. The blowout advancement in nanotechnology has urged the use of different types of fluorescent nanomaterials for hemoglobin assay. The past decades have witnessed the rapid progress of fluorescent nanosensors for hemoglobin assay. In the review, the sensing principles of fluorescent nanomaterials for sensing hemoglobin were briefly discussed. The advances of fluorescent nanosensors for detection of hemoglobin were further highlighted. And the sensing performance of fluorescent nanosensors versus traditional detection approaches was compared. Finally, the challenges and future directions of fluorescent nanomaterials for detection of hemoglobin are discussed. The review will arouse much more attention to the construction of hemoglobin sensors and facilitate rapid development of fluorescent nanosensors of hemoglobin.