Colorimetric Fluorescent Sensors for Hemoglobin Based on BODIPY Dyes.

Colorimetric fluorescent chemosensors 4 and 5 based on mono- and di- styryl borondipyrromethenes (BODIPY) linked methyl malonyl were designed for detection of hemoglobin (HgB). Their sensing behavior toward various analytes (Br, EDTA, Glucose, CO, Fe, Fe, I, NO, PO, SO,Cl, Urea, K, Mg, Na, NH, Zn,Ca,Cd, Li, Pb, Cs, Ag, Mn, Cr, Ni, Hg, Al) were investigated by fluorescence spectroscopies. Addition of HgB to acetone: water (4:1) solutions of BODIPYs 4 and 5 solutions gave visual color changes, as well as significantly quenched fluorescence emissions, while other analytes induced no or much smaller spectral changes. The sensing method for both BODIPYs 4 and 5 was successfully applied to measure the HgB in human blood with satisfactory results. Spike and recovery tests in human blood samples exhibit good recovery rates for the spiked concentrations close to the limit of detection. It was found that BODIPYs 4 and 5 constituted the HgB selective fluorescent chemosensor (ON-OFF) and the detection limits were calculated to be 1.773 μg. mL and 1. 295 μg.mL respectively.