College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan, China.
Academy of Medical Sciences, Zhengzhou University, Zhengzhou, Henan, China.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2021 Sep;13(5):e1716. doi: 10.1002/wnan.1716. Epub 2021 Mar 28.
Single-molecule detection represents the ultimate sensitivity in measurement science with the characteristics of simplicity, rapidity, low sample consumption, and high signal-to-noise ratio and has attracted considerable attentions in biosensor development. In recent years, a variety of functional nanomaterials with unique chemical, optical, mechanical, and electronic features have been synthesized. The integration of single-molecule detection with functional nanomaterials enables the construction of novel single-molecule fluorescent nanosensors with excellent performance. Herein, we review the advance in single-molecule fluorescent nanosensors constructed by novel nanomaterials including quantum dots, gold nanoparticles, upconversion nanoparticles, fluorescent conjugated polymer nanoparticles, nanosheets, and magnetic nanoparticles in the past decade (2011-2020), and discuss the strategies, features, and applications of single-molecule fluorescent nanosensors in the detection of microRNAs, DNAs, enzymes, proteins, viruses, and live cells. Moreover, we highlight the future direction and challenges in this area. This article is categorized under: Diagnostic Tools > Biosensing Diagnostic Tools > In Vitro Nanoparticle-Based Sensing Diagnostic Tools > Diagnostic Nanodevices.
单分子检测具有简单、快速、低样品消耗、高信噪比等特点，代表了测量科学的极限灵敏度，在生物传感器的发展中引起了相当多的关注。近年来，已合成了多种具有独特化学、光学、机械和电子特性的功能纳米材料。单分子检测与功能纳米材料的结合，使得构建具有优异性能的新型单分子荧光纳米传感器成为可能。本文综述了过去十年（2011-2020 年）利用新型纳米材料构建单分子荧光纳米传感器的进展，包括量子点、金纳米粒子、上转换纳米粒子、荧光共轭聚合物纳米粒子、纳米片和磁性纳米粒子，并讨论了单分子荧光纳米传感器在检测 microRNAs、DNAs、酶、蛋白质、病毒和活细胞中的策略、特点和应用。此外，我们还强调了该领域的未来方向和挑战。本文属于以下分类：诊断工具 > 生物传感诊断工具 > 基于纳米颗粒的体外检测诊断工具 > 诊断纳米器件。
