Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal-576104, India.
Centre for Applied Nanosciences, Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal-576104, India.
ACS Biomater Sci Eng. 2024 Feb 12;10(2):1153-1161. doi: 10.1021/acsbiomaterials.3c01275. Epub 2024 Jan 16.
Ultratrace elemental detections from a limited volume of samples can offer significant benefits in biomedical fields. However, it can be challenging to concentrate the particles being analyzed in a small area to improve the accuracy of detection. Ring-like deposits on the edges of colloidal droplets are a vexing problem in many applications. Herein, we report ultratrace elemental detection using a superhydrophobic surface-enhanced laser-induced breakdown spectroscopy (SELIBS) substrate fabricated by laser ablation followed by a soft lithography technique. In this work, the SELIBS spectra on a superhydrophobic polydimethylsiloxane (PDMS) substrate replicated from a laser-patterned master Teflon substrate are investigated. This work highlights the application of this newly created superhydrophobic substrate for detecting trace elements in body fluids using SELIBS. The developed PDMS substrate was successfully adopted to investigate the electrolyte variation in serum samples of oral cancer patients and normal volunteers. Principal component analysis (PCA) and match-no-match analysis were used to distinguish the elemental variation in cancer and control groups.
从有限体积的样品中进行超痕量元素检测，在生物医学领域具有重要意义。然而，要将被分析的颗粒集中到一个小区域以提高检测的准确性，这可能具有挑战性。胶体液滴边缘的环状沉积物是许多应用中的一个难题。在此，我们报告了使用超疏水表面增强激光诱导击穿光谱（SELIBS）衬底进行超痕量元素检测的方法，该衬底是通过激光烧蚀和软光刻技术制备的。在这项工作中，我们研究了从激光图案化的聚四氟乙烯（Teflon）母板复制的超疏水聚二甲基硅氧烷（PDMS）衬底上的 SELIBS 光谱。本工作重点介绍了使用 SELIBS 检测体液中痕量元素的这种新创建的超疏水衬底的应用。开发的 PDMS 衬底成功地用于研究口腔癌患者和正常志愿者血清样本中的电解质变化。主成分分析（PCA）和匹配-不匹配分析用于区分癌症组和对照组的元素变化。
