Biomaterials Research Center, School of Biomedical Engineering, Southern Medical University, Guangzhou 510515, China.
Division of Laboratory Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou 510282 China.
Nano Lett. 2024 Jan 31;24(4):1367-1375. doi: 10.1021/acs.nanolett.3c04483. Epub 2024 Jan 16.
Fluorescence imaging is a vital way to delineate the tumor boundaries. Here, we achieve a NIR-II aggregation-induced emission luminogen (AIEgen) with a fluorescence quantum yield (QY) of 12.6% in water through straightforward alkyl side chain modification. After loading of NIR-II AIEgen into polystyrene (PS) nanospheres, the thermal deactivation pathway is extremely limited, thereby concentrating absorption excitation on fluorescence emission. The fluorescence intensity is further enhanced by 5.4 times, the QY increases to 21.1%, and the NIR-II imaging signal is accordingly enhanced by 8.7 times, surpassing conventional DSPE-PEG carriers. The NIR-II@PS nanoprobe showcases superior resolution and tissue penetration depth compared to indocyanine green (ICG) and short-range near-infrared AIEgens. investigations underscore its tumor-to-normal tissue ratio (3.9) at 24 h post intravenous injection, enabling complete resection of ≤1 mm metastases under NIR-II bioimaging guidance. Additionally, the PS carrier-nanoparticles exhibit low toxicity , laying a promising foundation for the future design of medical nanomaterials.
荧光成像是描绘肿瘤边界的重要方法。在这里，我们通过简单的烷基侧链修饰，在水中实现了一种近红外二区聚集诱导发射发光体（AIEgen），其荧光量子产率（QY）为 12.6%。将近红外二区 AIEgen 负载到聚苯乙烯（PS）纳米球中后，热失活途径受到极大限制，从而将吸收激发集中在荧光发射上。荧光强度进一步增强了 5.4 倍，QY 增加到 21.1%，近红外二区成像信号相应增强了 8.7 倍，超过了传统的二硬脂酰基磷脂酰乙醇胺-聚乙二醇（DSPE-PEG）载体。与吲哚菁绿（ICG）和短程近红外 AIEgen 相比，近红外二区@PS 纳米探针具有更高的分辨率和组织穿透深度。研究强调了其在静脉注射后 24 小时的肿瘤与正常组织比值（3.9），能够在近红外二区生物成像引导下完全切除≤1mm 的转移灶。此外，PS 载体纳米颗粒表现出低毒性，为未来的医用纳米材料设计奠定了有前景的基础。
