Xu Da-Li, Zhang Xiao-Mei, Tian Xue-Ying, Wang Xian-Jun, Zhao Lin, Gao Meng-Ying, Li Lian-Feng, Zhao Jia-Qi, Cao Wu-Chun, Ding Shu-Jun
Department of Epidemiology, School of Public Health, Cheeloo College of Medicine, Shandong UniversityJinan, Shandong, People's Republic of China.
Shandong Provincial Key Laboratory of Communicable Disease Control and Prevention, Department of Communicable Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, Shandong Province, People's Republic of China.
J Inflamm Res. 2024 Jan 12;17:211-222. doi: 10.2147/JIR.S444398. eCollection 2024.
To characterize the cytokine profile of patients with severe fever with thrombocytopenia syndrome (SFTS) in relation to disease severity.
60 laboratory-confirmed SFTS patients and 12 healthy individuals from multi-centers in Shandong Province of China were included, and all patients were divided into fatal patients (9) and recovered patients (51) due to their final outcomes. Multiplex-microbead immunoassays were conducted to estimate levels of 27 cytokines in the sera of patients and controls.
The results showed that levels of IL-2, IL-4, IL-6, IL-7, IL-8, IL-15, IL-1RA, G-CSF, GM-CSF, IFN-γ, TNF-α, basic FGF, PDGF-BB, RANTES, IP-10, MIP-1α, MIP-1β, MCP-1, and Eotaxin differed significantly among the SFTS fatal patients, recovered patients, and the healthy controls (all <0.05). Compared to the healthy controls, the fatal patients and recovered patients had reduced levels of IL-2, IL-4, IL-7, PDGF-BB, RANTES, and Eotaxin, while the levels of PDGF-BB and RANTES were significantly lower in fatal patients compared to recovered patients. The increasing levels of IL-6, IL-8, IL-15, IL-1RA, G-CSF, GM-CSF, IFN-γ, TNF-α, basic FGF, IP-10, MIP-1α, MIP-1β, and MCP-1 were observed in fatal patients (all <0.05), and the levels of IL-6, IP-10, MIP-1α, and MCP-1 were significantly higher than other two groups. The Spearman correlation analysis indicated a positive correlation between platelet count and PDGF-BB levels (<0.05), while the white blood cell count had a negative correlation with MIP-1 level (<0.05).
The research exhibited that the SFTS virus (SFTSV) caused an atypical manifestation of cytokines. The levels of IL-6, IP-10, MIP-1α, and MCP-1 had been observed a positive association with the severity of the illness.
描述发热伴血小板减少综合征（SFTS）患者的细胞因子谱及其与疾病严重程度的关系。
纳入来自中国山东省多中心的60例实验室确诊的SFTS患者和12名健康个体，所有患者根据最终结局分为死亡患者（9例）和康复患者（51例）。采用多重微珠免疫测定法评估患者和对照组血清中27种细胞因子的水平。
结果显示，SFTS死亡患者、康复患者和健康对照组之间，IL-2、IL-4、IL-6、IL-7、IL-8、IL-15、IL-1RA、G-CSF、GM-CSF、IFN-γ、TNF-α、碱性成纤维细胞生长因子（basic FGF）、血小板源性生长因子BB（PDGF-BB）、调节激活正常T细胞表达和分泌因子（RANTES）、干扰素诱导蛋白10（IP-10）、巨噬细胞炎性蛋白1α（MIP-1α）、巨噬细胞炎性蛋白1β（MIP-1β）、单核细胞趋化蛋白1（MCP-1）和嗜酸性粒细胞趋化因子（Eotaxin）的水平差异均有统计学意义（均P<0.05）。与健康对照组相比，死亡患者和康复患者的IL-2、IL-4、IL-7、PDGF-BB、RANTES和Eotaxin水平降低，而死亡患者的PDGF-BB和RANTES水平显著低于康复患者。死亡患者中IL-6、IL-8、IL-15、IL-1RA、G-CSF、GM-CSF、IFN-γ、TNF-α、碱性成纤维细胞生长因子、IP-10、MIP-1α、MIP-1β和MCP-1水平升高（均P<0.05），且IL-6、IP-10、MIP-1α和MCP-1水平显著高于其他两组。Spearman相关性分析表明血小板计数与PDGF-BB水平呈正相关（P<0.05），而白细胞计数与MIP-1水平呈负相关（P<0.05）。
该研究表明，SFTS病毒（SFTSV）引起细胞因子的非典型表现。已观察到IL-6、IP-10、MIP-1α和MCP-1水平与疾病严重程度呈正相关。
