A rapid and specific antimicrobial resistance detection of magnetic nanoclusters.

Drinking water contamination, often caused by bacteria, leads to substantial numbers of diarrhea deaths each year, especially in developing regions. Human urine as a source of fertilizer, when handled improperly, can contaminate drinking water. One dominant bacterial pathogen in urine is , which can trigger serious waterborne/foodborne diseases. Considering the prevalence of the multi-drug resistant extended-spectrum beta-lactamase (ESBL) producing , a rapid detection method for resistance is highly desired. In this work, we developed a method for quick identification of and, at the same time, capable of removal of general bacterial pathogens from human urine. A specific peptide GRHIFWRRGGGHKVAPR, reported to have a strong affinity to , was utilized to modify the PEGylated magnetic nanoclusters, resulting in a specific capture and enrichment of from the bacteria-spiked artificial urine. Subsequently, a novel luminescent probe was applied to rapidly identify the antimicrobial resistance of the collected within 30 min. These functionalized magnetic nanoclusters demonstrate a promising prospect to rapidly detect ESBL in urine and contribute to reducing drinking water contamination.