Immunology Department, Institute of Biotechnology (IBT), Vietnam Academy of Science and Technology (VAST), 18. Hoang Quoc Viet Rd, Cau Giay, Hanoi, Vietnam.
University of Science and Technology of Hanoi (USTH), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet Rd, Cau Giay, Hanoi, Vietnam.
Parasitol Res. 2024 Jan 18;123(1):103. doi: 10.1007/s00436-023-08110-z.
Five newly obtained nuclear ribosomal transcription unit (rTU) sequences from Echinostomatidae and Echinochasmidae are presented. The inter- and intrafamilial relationships of these and other families in the suborder Echinostomata are also analyzed. The sequences obtained are the complete rTU of Artyfechinostomum malayanum (9,499 bp), the near-complete rTU of Hypoderaeum conoideum (8,076 bp), and the coding regions (from 5'-terminus of 18S to 3'-terminus of 28S rRNA gene) in Echinostoma revolutum (6,856 bp), Echinostoma miyagawai (6,854 bp), and Echinochasmus japonicus (7,150 bp). Except for the longer first internal transcribed spacer (ITS1) in Echinochasmus japonicus, all genes and spacers were almost identical in length. Comprehensive maximum-likelihood phylogenies were constructed using the PhyML software package. The datasets were either the concatenated 28S + 18S rDNA sequences (5.7-5.8 kb) from 60 complete rTUs of 19 families or complete 28S sequences only (about 3.8-3.9 kb) from 70 strains or species of 22 families. The phylogenetic trees confirmed Echinostomatoidea as monophyletic. Furthermore, a detailed phylogeny constructed from alignments of 169 28S D1-D3 rDNA sequences (1.1-1.3 kb) from 98 species of 50 genera of 10 families, including 154 echinostomatoid sequences (85 species/42 genera), clearly indicated known generic relationships within Echinostomatidae and Echinochasmidae and relationships of families within Echinostomata and several other suborders. Within Echinostomatidae, Echinostoma, Artyfechinostomum, and Hypoderaeum appeared as monophyletic, while Echinochasmus (Echinochasmidae) was polyphyletic. The Echinochasmidae are a sister group to the Psilostomidae. The datasets provided here will be useful for taxonomic reappraisal as well as studies of evolutionary and population genetics in the superfamily Echinostomatoidea, the sole superfamily in the suborder Echinostomata.
现提供从棘口科和碗口科获得的 5 个新的核核糖体转录单位（rTU）序列。还分析了这些序列以及其他亚目棘口类中的科内和科间的关系。获得的序列是完整的马来棘口吸虫（9499bp）rTU、近完整的 Hypoderaeum conoideum（8076bp）rTU 和编码区（从 18S 的 5'-末端到 28S rRNA 基因的 3'-末端）在旋转棘口吸虫（6856bp）、棘口吸虫（6854bp）和日本棘口吸虫（7150bp）。除了日本棘口吸虫较长的第一内部转录间隔子（ITS1）外，所有基因和间隔子的长度几乎相同。使用 PhyML 软件包构建了全面的最大似然系统发育。数据集要么是 19 个科的 60 个完整 rTU 的串联 28S + 18S rDNA 序列（5.7-5.8kb），要么是 70 个菌株或物种的完整 28S 序列（约 3.8-3.9kb）来自 22 个科。系统发育树证实棘口动物类是单系的。此外，从 50 个属的 98 种、10 个科的 169 个 28S D1-D3 rDNA 序列（1.1-1.3kb）构建的详细系统发育，包括 154 个棘口动物序列（85 种/42 属），清楚地表明了棘口科和碗口科内已知的属关系以及棘口类内的科间关系和其他几个亚目。在棘口科中，棘口属、Artyfechinostomum 和 Hypoderaeum 表现为单系，而 Echinochasmus（碗口科）是多系的。碗口科是 Psilostomidae 的姊妹群。提供的数据集将有助于分类学的重新评估以及在棘口动物超科（棘口类的唯一超科）的进化和种群遗传学研究。
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