Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathum Thani, 12121, Thailand.
Parasitol Res. 2013 Jan;112(1):437-41. doi: 10.1007/s00436-012-3065-9. Epub 2012 Aug 16.
Genetic differentiation between two synonymous echinostomes species, Artyfechinostomum malayanum and Artyfechinostomum sufrartyfex was determined by using the first and second internal transcribed spacers (ITS1 and ITS2), the non-coding region of rDNA as genetic makers. Of the 699 bp of combined ITS1 and ITS2 sequences examined, 18 variable nucleotide positions (2.58 %) were observed. Of these, 17 positions could be used as diagnostic position between these two sibling species, whereas the other one variation was intraspecific variation of A. malayanum. A clade of A. malayanum was closely aligned with A. sufrartyfex and clearly distance from the cluster of other echinostomes. Our results may sufficiently suggest that the current synonymy of these species is not valid.
利用第一和第二内部转录间隔区（ITS1 和 ITS2）以及 rDNA 的非编码区作为遗传标记，确定了两种同义的棘口吸虫（Artyfechinostomum malayanum 和 Artyfechinostomum sufrartyfex）之间的遗传分化。在检查的 699bp 联合 ITS1 和 ITS2 序列中，观察到 18 个可变核苷酸位置（2.58%）。其中，17 个位置可作为这两个姊妹种之间的诊断位置，而另一个变异是 A. malayanum 的种内变异。A. malayanum 的一个分支与 A. sufrartyfex 紧密对齐，并且明显与其他棘口吸虫的聚类分开。我们的结果足以表明，这些物种目前的同义并不成立。
