Department of Respiratory Medicine, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China.
Vision Medicals Center for Infectious Diseases, Guangzhou, 510705, China.
Respir Res. 2024 Jan 18;25(1):35. doi: 10.1186/s12931-024-02687-4.
This study aimed to investigate the interactions among three core elements of respiratory infection-pathogen, lung microbiome, and host response-and their avocation with the severity and outcomes of Mycoplasma pneumoniae pneumonia (MPP) in children.
We prospectively collected bronchoalveolar lavage fluid from a cohort of 41 children with MPP, including general MPP (GMPP) and complicated MPP (CMPP), followed by microbiome and transcriptomic analyses to characterize the association among pathogen, lung microbiome, and host response and correlate it with the clinical features and outcomes.
The lung microbiome of patients with CMPP had an increased relative abundance of Mycoplasma pneumoniae (MP) and reduced alpha diversity, with 76 differentially expressed species. Host gene analysis revealed a key module associated with neutrophil function and several inflammatory response pathways. Patients with a high relative abundance of MP, manifested by a specific lung microbiome and host response type, were more prone to CMPP and had a long imaging recovery time.
Patients with CMPP have a more disrupted lung microbiome than those with GMPP. MP, lung microbiome, and host response interacts with each other and are closely related to disease severity and outcomes in children with MPP.
本研究旨在探讨呼吸道感染病原体、肺部微生物组和宿主反应这三个核心要素之间的相互作用，以及它们与儿童肺炎支原体肺炎（MPP）严重程度和结局的关系。
我们前瞻性地收集了 41 例 MPP 患儿的支气管肺泡灌洗液，包括普通 MPP（GMPP）和复杂 MPP（CMPP），随后进行微生物组和转录组分析，以描述病原体、肺部微生物组和宿主反应之间的关联，并将其与临床特征和结局相关联。
CMPP 患儿的肺部微生物组中肺炎支原体（MP）的相对丰度增加，α多样性降低，有 76 个差异表达物种。宿主基因分析显示，一个与中性粒细胞功能和几个炎症反应途径相关的关键模块。具有特定肺部微生物组和宿主反应类型的高 MP 相对丰度的患者更容易发生 CMPP，并且影像学恢复时间较长。
与 GMPP 患儿相比，CMPP 患儿的肺部微生物组更为紊乱。MP、肺部微生物组和宿主反应相互作用，与儿童 MPP 的严重程度和结局密切相关。
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