The Lab for Research and Development of Actinomycete Resources, Yunnan Institute of Microbiology, Chenggong campus of Yunnan University, Kunming, 650500, PR China.
School of Marine Science and Biotechnology, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Guangxi University for Nationalities, Nanning 530008, PR China.
Int J Syst Evol Microbiol. 2024 Jan;74(1). doi: 10.1099/ijsem.0.006241.
A Gram-stain-negative, catalase-positive and oxidase-positive, nonmotile, aerobic, light yellow, spherical-shaped bacterial strain with no flagella, designated strain YIM 152171, was isolated from sediment of the South China Sea. Colonies were smooth and convex, light yellow and circular, and 1.0-1.5×1.0-1.5 µm in cell diameter after 7 days of incubation at 28°C on YIM38 media supplemented with sea salt. Colonies could grow at 20-45°C (optimum 28-35°C) and pH 6.0-11.0 (optimum, pH 7.0-9.0), and they could proliferate in the salinity range of 0-6.0 % (w/v) NaCl. The major cellular fatty acids were summed feature 8 (C 7/C ω6), C ω7 11-methyl, C, C ω11, C ω5, C ω6 and C ω5. The respiratory quinone was ubiquinone 10, and the polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol mannoside, one unidentified phospholipid and one unidentified aminolipid. Phylogenetic analyses based on the 16S rRNA gene sequences placed strain YIM 152171 within the order in a distinct lineage that also included the genus . The 16S rRNA gene sequence similarities of YIM 152171 to those of , and were 92.17, 89.25 and 88.91 %, respectively. The assembled draft genome of strain YIM 152171 had 136 contigs with an N50 value of 134704 nt, a total length of 3 001 346 bp and a G+C content of 70.27 mol%. The phylogenetic, phenotypic and chemotaxonomic data showed that strain YIM 152171 (=MCCC 1K08488=KCTC 92884) represents a type of novel species and genus for which we propose the name gen. nov., sp. nov.
一株革兰氏阴性、过氧化氢酶阳性和氧化酶阳性、非运动性、需氧、浅黄色、球形细菌菌株，无鞭毛，命名为 YIM 152171 菌株，从南海沉积物中分离得到。在 YIM38 培养基中添加海水盐，28°C 培养 7 天后，菌落光滑凸起，呈浅黄色，圆形，细胞直径为 1.0-1.5×1.0-1.5μm。菌落可在 20-45°C（最佳 28-35°C）和 pH6.0-11.0（最佳，pH7.0-9.0）生长，并可在 0-6.0%（w/v）NaCl 盐度范围内增殖。主要细胞脂肪酸为 sum feature 8（C7/Cω6）、Cω7 11-甲基、C、Cω11、Cω5、Cω6 和 Cω5。呼吸醌为泛醌 10，极性脂类谱包括双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、磷脂酰肌醇甘露糖苷、一种未鉴定的磷脂和一种未鉴定的胺脂。基于 16S rRNA 基因序列的系统发育分析将菌株 YIM 152171 置于目内的一个独特分支中，该分支还包括属。YIM 152171 与、和的 16S rRNA 基因序列相似性分别为 92.17%、89.25%和 88.91%。菌株 YIM 152171 的组装基因组草案有 136 个 contigs，N50 值为 134704nt，总长度为 3001346bp，G+C 含量为 70.27%mol%。系统发育、表型和化学分类学数据表明，菌株 YIM 152171（=MCCC 1K08488=KCTC 92884）代表一种新型的属和种，我们建议将其命名为新属、新种。
