Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources; State Key Laboratory Breeding Base of Marine Genetic Resources; Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China.
School of Environment, Harbin Institute of Technology, Harbin, PR China.
Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004790.
A novel Gram-stain-negative, facultatively anaerobic, rod-shaped bacterium, designated as D167-6-1, was isolated from deep-sea sediment collected from the Pacific Ocean. The cells were catalase- and oxidase-positive, and motile by means of peritrichous flagella. Growth occurred at NaCl concentrations ranging from 0 to 19 % (optimum, 2-8 %, w/v), from pH 6 to 11 (optimum, 7-8) and at temperatures between 4 and 45 °C (optimum, 33 °C). Phylogenetic analysis based on 16S rRNA, and gene sequences and its genome sequence revealed that strain D167-6-1 formed a monophyletic branch within the genus and was most closely related to , , , , and (with 98.5, 98.5, 98.4, 98.1, 97.5 and 97.8 % 16S rRNA sequence similarity, respectively). The complete genome size of strain D167-6-1 was 4.49 Mb, with a DNA G+C content of 62.8 mol%. The estimated averagenucleotide identity and DNA-DNA hybridization values between strain D167-6-1 and other closely related species were 77.59-85.35 % and 22.0-30.6 %, respectively. The principal cellular fatty acids (>5 %) were C 7, C, C cyclo 8, summed feature 3 (C 7/C 6) and C cyclo. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, an unidentified aminolipid, aminophospholipid and two unidentified phospholipids. The predominant respiratory quinones were Q-9 and Q-8. The combined genotypic and phenotypic data show that strain D167-6-1 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain D167-6-1 (=MCCC 1A13316=KCTC 72441).
一株新型革兰氏阴性、兼性厌氧、杆状细菌，被命名为 D167-6-1，从太平洋深海沉积物中分离得到。该细胞呈过氧化氢酶和氧化酶阳性，通过周生鞭毛运动。生长在 NaCl 浓度范围为 0-19%（最佳为 2-8%，w/v）、pH 值为 6-11（最佳为 7-8）和 4-45°C（最佳为 33°C）的环境中。基于 16S rRNA 基因和基因组序列的系统发育分析表明，菌株 D167-6-1在属内形成一个单系分支，与 、 、 、 、 和 （分别具有 98.5%、98.5%、98.4%、98.1%、97.5%和 97.8%的 16S rRNA 序列相似性）最为密切相关。菌株 D167-6-1 的完整基因组大小为 4.49Mb，DNA G+C 含量为 62.8mol%。菌株 D167-6-1 与其他密切相关物种的平均核苷酸同一性和 DNA-DNA 杂交值估计分别为 77.59-85.35%和 22.0-30.6%。主要的细胞脂肪酸（>5%）为 C 7、C 16:0、C 18:1 ω7c 和 C 18:1 cyclo。极性脂类鉴定为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰胆碱、一种未鉴定的胺脂、胺磷脂和两种未鉴定的磷脂。主要的呼吸醌为 Q-9 和 Q-8。综合表型和基因型数据表明，菌株 D167-6-1 代表属的一个新型种，建议命名为 sp. nov.，其模式菌株为 D167-6-1（=MCCC 1A13316=KCTC 72441）。
