College of Chemical Engineering and Materials Science, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.
Tianjin Key Laboratory of Brine Chemical Engineering and Resource Eco-Utilization, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China.
Extremophiles. 2024 Jan 19;28(1):11. doi: 10.1007/s00792-023-01328-w.
The isolated halophilic bacterial strain Halovibrio variabilis TG-5 showed a good performance in the pretreatment of coal gasification wastewater. With the optimum culture conditions of pH = 7, a temperature of 46 °C, and a salinity of 15%, the chemical oxygen demand and volatile phenol content of pretreated wastewater were decreased to 1721 mg/L and 94 mg/L, respectively. The removal rates of chemical oxygen demand and volatile phenol were over 90% and 70%, respectively. At the optimum salinity conditions of 15%, the total yield of intracellular compatible solutes and the extracellular transient released yield under hypotonic conditions were increased to 6.88 g/L and 3.45 g/L, respectively. The essential compatible solutes such as L-lysine, L-valine, and betaine were important in flocculation mechanism in wastewater pretreatment. This study provided a new method for pretreating coal gasification wastewater by halophilic microorganisms, and revealed the crucial roles of compatible solutes in the flocculation process.
一株分离得到的嗜盐菌 Halovibrio variabilis TG-5 在煤气化废水预处理中表现出良好的性能。在最佳培养条件下（pH=7、温度 46°C、盐度 15%），预处理后废水中的化学需氧量和挥发酚含量分别降低至 1721mg/L 和 94mg/L，去除率均超过 90%和 70%。在最佳盐度 15%条件下，胞内相容性溶质的总产率和在低渗条件下细胞外瞬时释放产量分别增加至 6.88g/L 和 3.45g/L。L-赖氨酸、L-缬氨酸和甜菜碱等必需相容性溶质在废水预处理中的絮凝机制中发挥了重要作用。本研究为嗜盐微生物预处理煤气化废水提供了一种新方法，并揭示了相容性溶质在絮凝过程中的关键作用。
