Sagisaka K, Noguchi M, Funayama M, Aoki Y
Tohoku J Exp Med. 1986 Nov;150(3):345-51. doi: 10.1620/tjem.150.345.
Immune agglutinin or precipitin loses its reactivity with the corresponding antigen by heating or some chemicals. A. Se group saliva or M group blood stain was sensitized with human IgM anti-A or rabbit IgG anti-M serum, respectively. After being washed with saline, the stains were heated or treated with formalin or 2-mercaptoethanol (2-ME) of various concentrations. The immunoreactivity of the antibodies in the state of immunecomplex was examined by the mixed agglutination or elution test, indicating IgM and IgG antibodies in the state of immunecomplex were proof against heating up to 150 degrees C for 10 min and against formalin and 2-ME of high concentrations. It is considered that IgM and IgG acquire a new property of heat and chemical stability by formation of immunecomplex although they are substantially heat and chemical labile.
免疫凝集素或沉淀素通过加热或某些化学物质会失去与相应抗原的反应性。分别用人IgM抗 - A血清或兔IgG抗 - M血清致敏A.Se组唾液或M组血迹。用盐水洗涤后，对污渍进行加热或用不同浓度的福尔马林或2 - 巯基乙醇（2 - ME）处理。通过混合凝集试验或洗脱试验检测免疫复合物状态下抗体的免疫反应性，结果表明免疫复合物状态下的IgM和IgG抗体能耐150摄氏度加热10分钟以及高浓度的福尔马林和2 - ME。据认为，IgM和IgG虽然本质上对热和化学物质不稳定，但通过形成免疫复合物获得了热和化学稳定性的新特性。
