Kim Y D, Prakash U, Weber G F, Hargie M
Immunol Commun. 1979;8(4):397-406. doi: 10.3109/08820137909050053.
Antibodies against blood group substance T in normal human sera consist of two types, depending upon whether or not the molecules remain immunochemically active at 37 degrees C. The two agglutinins were isolated by means of T antigen-coupled Sepharose 48 affinity chromatography. Temperature-sensitive agglutinin was eluted from the affinity column at 37 degrees C, while temperature-independent agglutinin remained bound to the antigen. Subsequently, the latter was dissociated from the column in the presence of 2.5 M MgCl2. Examination of six normal sera revealed that the levels of temperature-independent agglutinin was about twice that of temperature-sensitive agglutinin. More than 90% of anti-T agglutinins in normal sera were of the IgM class, in 8 of 11 samples studied. No direct relationship appeared to exist between anti-T titers and blood type. Thermal effects on hemagglutination, at least for anti-T agglutinin interacting with T antigen bearing red cells, is primarily due to the molecular characteristics of the agglutinin and not to the conformational change of the red cell membranes.
正常人血清中抗血型物质T的抗体有两种类型，这取决于分子在37℃时是否仍保持免疫化学活性。通过T抗原偶联的琼脂糖4B亲和层析法分离出这两种凝集素。温度敏感凝集素在37℃时从亲和柱上洗脱下来，而温度不依赖凝集素仍与抗原结合。随后，在2.5M MgCl₂存在的情况下，后者从柱上解离。对6份正常人血清的检测显示，温度不依赖凝集素的水平约为温度敏感凝集素的两倍。在所研究的11个样本中的8个样本中，正常人血清中超过90%的抗-T凝集素属于IgM类。抗-T滴度与血型之间似乎没有直接关系。热对血凝的影响，至少对于与携带T抗原的红细胞相互作用的抗-T凝集素来说，主要是由于凝集素的分子特性，而不是由于红细胞膜的构象变化。
