Division of Neonatology, Department of Pediatrics, Shenzhen People's Hospital, The Second Clinical Medical College of Jinan University, First Affiliated Hospital of Southern University of Science and Technology, Shenzhen, 518020, China.
Department of Pediatrics, Nanjing Maternity and Child Health Care Hospital, Women's Hospital of Nanjing Medical University, Nanjing, 210004, China.
J Bioenerg Biomembr. 2024 Apr;56(2):171-180. doi: 10.1007/s10863-024-10002-5. Epub 2024 Jan 20.
To verify the protective effect of circDNAJB6 on Bronchopulmonary dysplasia (BPD) cell and animal models and to explore the possible mechanism of its protective effect. The function of circDNAJB6 was investigated at the cell and animal levels. Nuclear and Cytoplasmic RNA extraction kits and fluorescence in situ hybridization (FISH) were used to explore the distribution of circDNAJB6 in cells, and the potential mechanism of circDNAJB6 was verified by q-PCR, luciferase assays and rescue experiments.CircDNAJB6 is abundant in breast milk exosomes. Overexpression of circDNAJB6 can ameliorate damage in BPD models caused by hyperoxia exposure in vivo and in vitro. Mechanistically, circDNAJB6 can target the downstream DNAJB6 gene and promote the transcription of DNAJB6, exertive a protective effect on the experimental BPD model. Our results showed that circDNAJB6 alleviated damage and inhibited the proliferation of alveolar epithelial cells in the BPD model by promoting transcription of parent gene DNAJB6. Human milk exosome-derived circDNAJB6 provides new directions for preventing and treating BPD.
为了验证环状 DNAJB6 对支气管肺发育不良（BPD）细胞和动物模型的保护作用，并探讨其保护作用的可能机制。在细胞和动物水平上研究了 circDNAJB6 的功能。使用核和细胞质 RNA 提取试剂盒和荧光原位杂交（FISH）来探索 circDNAJB6 在细胞中的分布，通过 q-PCR、荧光素酶测定和挽救实验验证 circDNAJB6 的潜在机制。环状 DNAJB6 在母乳外泌体中含量丰富。circDNAJB6 的过表达可以改善体内和体外高氧暴露引起的 BPD 模型的损伤。在机制上，circDNAJB6 可以靶向下游的 DNAJB6 基因，并促进 DNAJB6 的转录，对实验性 BPD 模型发挥保护作用。我们的结果表明，circDNAJB6 通过促进亲本基因 DNAJB6 的转录，减轻 BPD 模型中的损伤并抑制肺泡上皮细胞的增殖。人乳外泌体衍生的 circDNAJB6 为预防和治疗 BPD 提供了新的方向。
