应用下一代测序技术研究支气管肺发育不良早产儿外周血环状 RNA 谱。
Next-generation sequencing to investigate circular RNA profiles in the peripheral blood of preterm neonates with bronchopulmonary dysplasia.
机构信息
Department of Neonates, Children's Hospital of Nanjing Medical University, Nanjing, China.
Department of Immunology, National Health Commission of Key Lab of Antibody Technology, Nanjing Medical University, Nanjing, China.
出版信息
J Clin Lab Anal. 2020 Jul;34(7):e23260. doi: 10.1002/jcla.23260. Epub 2020 Feb 24.
BACKGROUND
Circular RNAs (circRNAs) are emerging noncoding RNAs that are involved in many biological processes and diseases. The expression profile of circRNAs in preterm neonates with bronchopulmonary dysplasia (BPD) remains unresolved.
METHODS
In BPD infants, peripheral venous blood was drawn and circRNAs were extracted and sequenced by next-generation sequencing. The levels of the selected circRNAs were measured by real-time quantitative reverse transcription PCR.
RESULTS
Among thousands of circRNAs, 491 circRNAs were significantly changed. Among the top 10 changed circRNAs, hsa_circ_0003122, hsa_circ_0003357, hsa_circ_0009983, hsa_circ_0003037, and hsa_circ_0009256 were significantly increased, while hsa_circ_0014932, hsa_circ_0015109, hsa_circ_0017811, hsa_circ_0020588, and hsa_circ_0015066 were significantly decreased. These altered circRNAs are involved in complicated biological functions and signaling pathways. Additionally, hsa_circ_0005577 (hsa_circ_FANCL), which was significantly increased in the moderate-to-severe BPD subjects, was correlated with oxygenation therapy.
CONCLUSION
These results suggest that an aberrant circRNA profile in the peripheral blood of BPD infants might be important in BPD pathogenesis.
背景
环状 RNA（circRNA）是新兴的非编码 RNA，参与多种生物学过程和疾病。支气管肺发育不良（BPD）早产儿中 circRNA 的表达谱仍未得到解决。
方法
在 BPD 婴儿中，采集外周静脉血，通过下一代测序提取和测序 circRNA。通过实时定量逆转录 PCR 测量选定 circRNA 的水平。
结果
在数千个 circRNA 中，有 491 个 circRNA 发生了显著变化。在 top10 变化的 circRNAs 中，hsa_circ_0003122、hsa_circ_0003357、hsa_circ_0009983、hsa_circ_0003037 和 hsa_circ_0009256 显著上调，而 hsa_circ_0014932、hsa_circ_0015109、hsa_circ_0017811、hsa_circ_0020588 和 hsa_circ_0015066 显著下调。这些改变的 circRNAs 涉及复杂的生物学功能和信号通路。此外，在中重度 BPD 患者中显著增加的 hsa_circ_0005577（hsa_circ_FANCL）与氧疗相关。
结论
这些结果表明，BPD 婴儿外周血中异常的 circRNA 谱可能在 BPD 发病机制中很重要。
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