Fu Zhenlan, Li Qingqing, Jiang Peiyao, Song Xiongbo, Yang Junjun, Chen Guangxing, Gong Xiaoyuan, Yang Liu
Center for Joint Surgery, Southwest Hospital, Army Medical University (Third Military Medical University), Chongqing, 400038, China.
Key Laboratory of Biorheological Science and Technology, Ministry of Education College of Bioengineering, Chongqing University, Chongqing, 400044, China.
Mol Biol Rep. 2024 Jan 21;51(1):154. doi: 10.1007/s11033-023-09101-0.
The senescence of chondrocytes, which is closely linked to the development of osteoarthritis (OA), has been found to be influenced by the inflammatory environment of joint cavity. However, there remains a lack of comprehensive understanding regarding the specific mechanisms through which cytokine impacts chondrocytes senescence.
To investigate the effects of MIF on the chondrocytes senescence and explore the underlying mechanism.
Human cytokine array and ELISA were used for the level of MIF in synovium fluid. CCK-8 was used for chondrocytes viability. IF, WB, SA-β-gal staining and flow cytometry were used for the chondrogenic, apoptotic and senescent phenotype of chondrocytes.
The level of MIF was significantly increased in OA patients. MIF significantly reversed the senescent phenotype induced by LPS pretreatment in human chondrocytes. MIF significantly enhanced the expression of Col II, SOX9, and ACAN in LPS pre-treated human chondrocytes. Furthermore, MIF significantly inhibited the apoptosis of LPS-induced senescent chondrocytes.
Increased level of MIF in osteoarthritic joint cavity might effectively suppress the senescent phenotype and simultaneously improve the chondrogenic phenotype in chondrocytes, the underlying mechanism was likely to be independent of apoptosis.
软骨细胞衰老与骨关节炎（OA）的发展密切相关，已发现其受关节腔炎症环境影响。然而，关于细胞因子影响软骨细胞衰老的具体机制仍缺乏全面了解。
研究巨噬细胞移动抑制因子（MIF）对软骨细胞衰老的影响并探讨其潜在机制。
用人细胞因子阵列和酶联免疫吸附测定法检测滑液中MIF水平。用细胞计数试剂盒-8（CCK-8）检测软骨细胞活力。用免疫荧光（IF）、蛋白质免疫印迹法（WB）、衰老相关β-半乳糖苷酶（SA-β-gal）染色和流式细胞术检测软骨细胞的成软骨、凋亡和衰老表型。
OA患者的MIF水平显著升高。MIF显著逆转了脂多糖（LPS）预处理诱导的人软骨细胞衰老表型。MIF显著增强了LPS预处理的人软骨细胞中II型胶原（Col II）、SRY-box转录因子9（SOX9）和聚集蛋白聚糖（ACAN）的表达。此外，MIF显著抑制了LPS诱导的衰老软骨细胞凋亡。
骨关节炎关节腔中MIF水平升高可能有效抑制软骨细胞衰老表型，同时改善其成软骨表型，其潜在机制可能与凋亡无关。
