State Key Laboratory of Radiation Medicine and Protection, School for Radiological and Interdisciplinary Sciences (RAD-X), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China.
School of Nuclear Science and Technology, University of Science and Technology of China, Hefei, 230026, China.
Angew Chem Int Ed Engl. 2024 Mar 11;63(11):e202318545. doi: 10.1002/anie.202318545. Epub 2024 Feb 6.
Afterglow imaging holds great promise for ultrasensitive bioimaging due to its elimination of autofluorescence. Self-sustaining afterglow molecules (SAMs), which enable all-in-one photon sensitization, chemical defect formation and afterglow generation, possess a simplified, reproducible, and efficient superiority over commonly used multi-component systems. However, there is a lack of SAMs, particularly those with much brighter near-infrared (NIR) emission and structural flexibility for building high-contrast activatable imaging probes. To address these issues, this study for the first time reports a methylene blue derivative-based self-sustaining afterglow agent (SAN-M) with brighter NIR afterglow chemiluminescence peaking at 710 nm. By leveraging the structural flexibility and tunability, an activatable nanoprobe (SAN-MO) is customized for simultaneously activatable fluoro-photoacoustic and afterglow imaging of peroxynitrite (ONOO ), notably with a superior activation ratio of 4523 in the afterglow mode, which is at least an order of magnitude higher than other reported activatable afterglow systems. By virtue of the elimination of autofluorescence and ultrahigh activation contrast, SAN-MO enables early monitoring of the LPS-induced acute inflammatory response within 30 min upon LPS stimulation and precise image-guided resection of tiny metastatic tumors, which is unattainable for fluorescence imaging.
余晖成像是一种超灵敏生物成像技术，因为它消除了自发荧光。自持续余晖分子（SAM）能够实现一体化的光子敏化、化学缺陷形成和余晖产生，相比于常用的多组分系统具有简化、可重复和高效的优势。然而，SAM 的应用仍然存在一些限制，特别是在近红外（NIR）发射强度和结构灵活性方面，这对于构建高对比度的激活型成像探针非常重要。为了解决这些问题，本研究首次报道了一种基于亚甲基蓝的自持续余晖试剂（SAN-M），它具有更亮的 NIR 余晖化学发光，峰值在 710nm。通过利用结构的灵活性和可调性，定制了一种可激活的纳米探针（SAN-MO），用于同时激活氟荧光声动力学和过氧亚硝酸盐（ONOO）的余晖成像，在余晖模式下的激活比率高达 4523，比其他报道的可激活余晖系统至少高出一个数量级。由于消除了自发荧光和超高的激活对比度，SAN-MO 能够在 LPS 刺激后 30 分钟内早期监测 LPS 诱导的急性炎症反应，并进行精确的图像引导切除微小的转移性肿瘤，这是荧光成像无法实现的。
