Heyman Y, Vincent C, Garnier V, Cognie Y
Vet Rec. 1987 Jan 24;120(4):83-5. doi: 10.1136/vr.120.4.83.
Embryos collected from ewes six days after oestrus were frozen in straws using ethylene-glycol as a cryoprotectant. The efficiency of the simplified freezing and thawing procedure was assessed after transfer, which resulted in an overall survival rate of 58.3 per cent. Forty-two lambs were born from 72 frozen embryos which had been transferred without any attempt to evaluate them after the thawing and sucrose dilution process.
在发情期后六天从母羊采集的胚胎，使用乙二醇作为冷冻保护剂，以细管进行冷冻。在胚胎移植后评估了简化的冻融程序的效率，总体存活率为58.3%。72枚冷冻胚胎在解冻和蔗糖稀释后未经任何评估便进行移植，最终产下了42只羔羊。
