Lehn-Jensen H
Nord Vet Med. 1981 Sep-Nov;33(9-11):476-83.
The original methods of storage of cow blastocysts involved slow freezing down to -60 degrees C combined with slow thawing. However, short freezing curves with plunge temperatures between -30 to -40 degrees C will give high survival/pregnancy rates when rapid thawing is applied, i.e. direct transfer into water at 37 degrees C. Early reports on freezing of mammalian embryos utilized DMSO as a cryoprotectant, but recent work seems to indicate that glycerol might be marginally better than DMSO in work with cow embryos. Seventeen out of 29 (59%) non-surgically transferred deep-frozen thawed embryos resulted in established pregnancies (greater than 60 days) (Table I:12) indicating that deep-freezing of cattle blastocysts when optimally applied only lowers results marginally compared to direct transfer.
牛囊胚的原始保存方法包括缓慢冷冻至-60摄氏度并结合缓慢解冻。然而，当采用快速解冻（即直接转移到37摄氏度的水中）时，骤冷温度在-30至-40摄氏度之间的短冷冻曲线将产生较高的存活/妊娠率。早期关于哺乳动物胚胎冷冻的报告使用二甲基亚砜（DMSO）作为冷冻保护剂，但最近的研究似乎表明，在处理牛胚胎时，甘油可能略优于DMSO。29个经非手术移植的深度冷冻解冻胚胎中有17个（59%）成功妊娠（超过60天）（表I:12），这表明在最佳应用条件下，牛囊胚的深度冷冻与直接移植相比，结果仅略有降低。
