Yoshikawa T, Flory W, Ruhr L P, Giamalva D, Church D F, Pryor W A
Vet Hum Toxicol. 1987 Feb;29(1):25-30.
Male Sprague-Dawley rats were treated with a single ip injection of dimethyl sulfoxide (DMSO), phenanthrene, nitrated products of phenanthrene, pyrene, or nitrated products of pyrene. Phenanthrene, pyrene and their nitrated products were dissolved in DMSO. Phenanthrene produced a significant elevation of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels relative to DMSO-injected rats 24 hr after injection. Gamma-glutamyl transpeptidase (GGTP) levels were significantly increased for groups treated with phenanthrene when compared with the DMSO group 72 hr after injection. Nitrated products of phenanthrene produced a significant elevation of serum AST, ALT, sorbitol dehydrogenase (SDH), and GGTP levels when compared with groups treated with DMSO and phenanthrene 24 hr after injection. Four of six rats in the nitrated phenanthrene treatment group died between 48 and 72 hr after the injection. Injection of pyrene caused no significant increases in serum enzyme activities. Significant changes in the serum AST, SDH and LDH levels were observed with the nitrated products of pyrene at 24 hr. Only SDH levels were significantly different when pyrene and its nitrated products were compared. No significant differences were detected at 72 hr with the nitrated products of pyrene. As supported by serum chemistry, this study suggests that the products of the reaction of NO2 with two model polynuclear aromatic hydrocarbons (PAH) are hepatotoxic. Both pyrene and phenanthrene form nitrated products that are more toxic than the parent PAH, but the nitrated products of phenanthrene appear to be more toxic than the nitration products of pyrene.
对雄性斯普拉格-道利大鼠进行腹腔注射，分别给予二甲基亚砜（DMSO）、菲、菲的硝化产物、芘或芘的硝化产物。菲、芘及其硝化产物均溶于DMSO。注射后24小时，与注射DMSO的大鼠相比，菲使血清天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平显著升高。注射72小时后，与DMSO组相比，用菲处理的组γ-谷氨酰转肽酶（GGTP）水平显著升高。与注射DMSO和菲的组相比，注射后24小时，菲的硝化产物使血清AST、ALT、山梨醇脱氢酶（SDH）和GGTP水平显著升高。在菲的硝化产物处理组中，6只大鼠中有4只在注射后48至72小时死亡。注射芘未引起血清酶活性显著增加。注射后24小时，芘的硝化产物使血清AST、SDH和乳酸脱氢酶（LDH）水平发生显著变化。当比较芘及其硝化产物时，只有SDH水平存在显著差异。注射后72小时，芘的硝化产物未检测到显著差异。血清化学分析结果表明，本研究提示二氧化氮与两种典型多环芳烃（PAH）反应的产物具有肝毒性。芘和菲均可形成毒性比母体PAH更强的硝化产物，但菲的硝化产物似乎比芘的硝化产物毒性更强。
