Yoshikawa T, Ruhr L P, Flory W, Giamalva D, Church D F, Pryor W A
Toxicol Appl Pharmacol. 1985 Jun 30;79(2):218-26. doi: 10.1016/0041-008x(85)90343-6.
Male Sprague-Dawley rats were treated with a single ip injection of physiological saline (3.0 ml/kg), dimethyl sulfoxide (DMSO, 3.0 ml/kg), phenanthrene (150 mg/kg), ozonized products of phenanthrene (150 mg/kg), pyrene (150 mg/kg), or ozonized products of pyrene (150 mg/kg). Phenanthrene, pyrene, and their ozonized products were dissolved in DMSO (50 mg/ml). Serum aspartate aminotransferase (AST) activity was increased significantly 24 hr after ip administration of DMSO when compared with physiological saline. Phenanthrene produced a significant elevation of serum AST and gamma-glutamyl transpeptidase (GGTP) levels related to physiological saline and DMSO-injected rats 24 hr after injection. However, GGTP levels for groups treated with DMSO or phenanthrene were not significantly increased when compared with saline groups 72 hr after injection. Ozonized products of phenanthrene produced a significant elevation of serum AST, alanine aminotransferase (ALT), GGTP, and bilirubin levels when compared with groups treated with physiological saline, DMSO, and phenanthrene 24 or 72 hr after injections. The ozonized products of phenanthrene also produced significant elevation of serum creatinine levels compared with physiological saline, DMSO, and phenanthrene groups at 24 hr after treatment and of blood urea nitrogen (BUN) levels at 24 and 72 hr. Although pyrene caused a small but significant increase in the serum AST and bilirubin levels 24 hr after treatment, no significant change in the serum AST, ALT, GGTP, BUN, and creatine levels were observed with the ozonized products of pyrene at 24 or 72 hr. This study demonstrates significant alterations in serum chemistry induced by reaction products of ozone with phenanthrene. No such effect was observed when the products of pyrene ozonation were administered. Although the ozonation products of pyrene were not toxic under the conditions of this study, phenanthrene products were more hepatotoxic than was phenanthrene itself. Nephrotoxicity was also an apparent effect of ozonized phenanthrene. Since ozone-polycyclic aromatic hydrocarbon (PAH) reactions may occur in the atmosphere, these reactions might produce compounds that are more toxic than either ozone or the PAH alone.
将雄性Sprague-Dawley大鼠通过腹腔注射给予单次剂量的生理盐水（3.0毫升/千克）、二甲基亚砜（DMSO，3.0毫升/千克）、菲（150毫克/千克）、菲的臭氧化产物（150毫克/千克）、芘（150毫克/千克）或芘的臭氧化产物（150毫克/千克）。菲、芘及其臭氧化产物均溶解于DMSO（50毫克/毫升）中。与生理盐水组相比，腹腔注射DMSO后24小时，血清天冬氨酸氨基转移酶（AST）活性显著升高。注射后24小时，与生理盐水组和注射DMSO的大鼠相比，菲使血清AST和γ-谷氨酰转肽酶（GGTP）水平显著升高。然而，注射72小时后，与生理盐水组相比，用DMSO或菲处理的组的GGTP水平没有显著升高。与注射生理盐水、DMSO和菲的组相比，注射菲的臭氧化产物后24或72小时，血清AST、丙氨酸氨基转移酶（ALT）、GGTP和胆红素水平显著升高。与生理盐水、DMSO和菲组相比，处理后24小时，菲的臭氧化产物还使血清肌酐水平显著升高，24和72小时时使血尿素氮（BUN）水平显著升高。尽管芘在处理后24小时使血清AST和胆红素水平有小幅但显著的升高，但芘的臭氧化产物在24或72小时时，血清AST、ALT、GGTP、BUN和肌酐水平未观察到显著变化。本研究表明，臭氧与菲的反应产物可引起血清化学指标的显著改变。给予芘的臭氧化产物时未观察到此类效应。尽管在本研究条件下芘的臭氧化产物无毒，但菲的产物比菲本身的肝毒性更强。肾毒性也是菲臭氧化产物的明显效应。由于臭氧与多环芳烃（PAH）的反应可能在大气中发生，这些反应可能产生比单独的臭氧或PAH毒性更强的化合物。
