Liu Yu, Tao Yi-Dong, Zhang Li-Bao, Wang Fen, Xu Jin, Zhang Jun-Zhong, Fu Da-Ying
Key Laboratory of Forest Disaster Warning and Control in Yunnan Province, Faculty of Biodiversity Conservation, Southwest Forestry University, Kunming 650224, China.
Yunnan Key Laboratory of Plateau Wetland Conservation, Restoration and Ecological Services, Southwest Forestry University, Kunming 650224, China.
Insects. 2023 Dec 26;15(1):10. doi: 10.3390/insects15010010.
In the present study, we found that blue light stress negatively affected the development periods, body weight, survival and reproduction of , and it showed a dose-dependent reaction, as longer irradiation caused severer effects. Further transcriptome analysis found blue light stress induced fast and large-scale transcriptional changes in the head, thorax and, particularly, the abdomen of female adults. A functional enrichment analysis indicated that shorter durations of blue light irradiation induced the upregulation of more stress response- and defense-related genes or pathways, such as abiotic stimuli detection and response, oxidative stress, ion channels and protein-kinase-based signal pathways. In the abdomen, however, different durations of blue-light-exposure treatments all induced the downregulation of a large number genes and pathways related to cellular processes, metabolism, catalysis and reproduction, which may be a trade-off between antistress defense and other processes or a strategy to escape stressful conditions. These results indicate irradiation duration- and tissue-specific blue light stress responses and consequences, as well as suggest that the stress that results in transcriptional alterations is associated with the stress that causes a fitness reduction in females.
在本研究中，我们发现蓝光胁迫对[物种名称]的发育时期、体重、存活和繁殖产生负面影响，且呈现剂量依赖性反应，即照射时间越长，影响越严重。进一步的转录组分析发现，蓝光胁迫在雌性[物种名称]成虫的头部、胸部，尤其是腹部诱导了快速且大规模的转录变化。功能富集分析表明，较短时间的蓝光照射诱导了更多与应激反应和防御相关的基因或通路的上调，如非生物刺激检测与反应、氧化应激、离子通道和基于蛋白激酶的信号通路。然而，在腹部，不同时长的蓝光暴露处理均诱导了大量与细胞过程、代谢、催化和繁殖相关的基因和通路的下调，这可能是抗应激防御与其他过程之间的一种权衡，或是逃避应激条件的一种策略。这些结果表明了照射时长和组织特异性的蓝光应激反应及后果，同时也表明导致转录改变的应激与导致雌性[物种名称]适合度降低的应激相关。
