Balakireva Yevgenia, Nikitina Maria, Makhnovskii Pavel, Kukushkina Inna, Kuzmin Ilya, Kim Alexander, Nefedova Lidia
Department of Genetics, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia.
Institute of Biomedical Problems, Russian Academy of Sciences, 123007 Moscow, Russia.
Insects. 2024 Jan 17;15(1):68. doi: 10.3390/insects15010068.
(1) Background: The gene in 's genome originated from the molecular domestication of retrotransposons and retroviruses' gene. In all species, the Gagr protein homologs exhibit a conserved structure, indicative of a vital role. Previous studies have suggested a potential link between the gene function and stress responses. (2) Methods: We compared flies with gene knockdown in all tissues to control flies in physiological tests and RNA-sequencing experiments. (3) Results: Flies with the gene knockdown exhibited shorter lifespans compared to control flies. Transcriptome analysis revealed that knockdown flies showed elevated transcription levels of immune response genes. We used ammonium persulfate, a potent stress inducer, to elicit a stress response. In control flies, ammonium persulfate activated the Toll, JAK/STAT, and JNK/MAPK signaling pathways. In contrast, flies with the gene knockdown displayed reduced expression of stress response genes. Gene ontology enrichment analysis identified categories of genes upregulated under ammonium persulfate stress in control flies but not in knockdown flies. These genes are involved in developmental control, morphogenesis, and central nervous system function. (4) Conclusion: Our findings indicate the significance of the gene in maintaining immune response and homeostasis.
(1) 背景：[物种]基因组中的[基因名称]基因起源于逆转座子和逆转录病毒的[相关基因名称]基因的分子驯化。在所有[物种名称]物种中，Gagr蛋白同源物呈现出保守结构，表明其具有重要作用。先前的研究表明[基因名称]基因功能与应激反应之间存在潜在联系。(2) 方法：我们在生理测试和RNA测序实验中，将所有组织中[基因名称]基因敲低的果蝇与对照果蝇进行比较。(3) 结果：与对照果蝇相比，[基因名称]基因敲低的果蝇寿命较短。转录组分析显示，[基因名称]基因敲低的果蝇免疫反应基因转录水平升高。我们使用强力应激诱导剂过硫酸铵来引发应激反应。在对照果蝇中，过硫酸铵激活了Toll、JAK/STAT和JNK/MAPK信号通路。相比之下，[基因名称]基因敲低的果蝇应激反应基因表达降低。基因本体富集分析确定了在对照果蝇中过硫酸铵应激下上调但在[基因名称]基因敲低果蝇中未上调的基因类别。这些基因参与发育控制、形态发生和中枢神经系统功能。(4) 结论：我们的研究结果表明[基因名称]基因在维持免疫反应和体内平衡中的重要性。
