Ghasemieshkaftaki Maryam, Cao Trung, Hossain Ahmed, Vasquez Ignacio, Santander Javier
Marine Microbial Pathogenesis and Vaccinology Laboratory, Department of Ocean Sciences, Memorial University of Newfoundland, St. John's, NL A1C 5S7, Canada.
Vaccines (Basel). 2024 Jan 10;12(1):70. doi: 10.3390/vaccines12010070.
Winter ulcer disease is a health issue in the Atlantic salmonid aquaculture industry, mainly caused by . Although vaccination is one of the effective ways to prevent bacterial outbreaks in the salmon farming industry, ulcer disease related to bacterial infections is being reported on Canada's Atlantic coast. Here, we studied the immune response of farmed immunized Atlantic salmon to bath and intraperitoneal (ip) challenges and evaluated the immunogenicity of cell components. IgM titers were determined after infection, post boost immunization, and post challenge with . IgM (B cell) in the spleen and blood cell populations were also identified and quantified by 3,3 dihexyloxacarbocyanine (DiOC6) and IgM-Texas red using confocal microscopy and flow cytometry. At 14 days post challenge, IgM was detected in the serum and spleen. There was a significant increase in circulating neutrophils 3 days after ip and bath challenges in the outer membrane vesicles (OMVs) boosted group compared to non-boosted. Lymphocytes increased in the blood at 7 and 14 days after the ip and bath challenges, respectively, in OMVs boosted group. Furthermore, a rise in IgM titers was detected in the OMVs boosted group. We determined that a commercial vaccine is effective against strain, and OMVs are the most immunogenic component of cells.
冬季溃疡病是大西洋鲑鱼养殖行业中的一个健康问题，主要由 引起。尽管疫苗接种是预防鲑鱼养殖行业细菌爆发的有效方法之一，但加拿大的大西洋沿岸仍有与细菌感染相关的溃疡病报告。在此，我们研究了养殖的免疫大西洋鲑鱼对浸浴和腹腔内（ip）攻击的免疫反应，并评估了 细胞成分的免疫原性。在感染后、加强免疫后以及用 攻击后测定IgM滴度。还使用共聚焦显微镜和流式细胞术通过3,3-二己基氧杂碳菁（DiOC6）和IgM-德克萨斯红对脾脏和血细胞群体中的IgM（B细胞）进行了鉴定和定量。在攻击后14天，在血清和脾脏中检测到IgM。与未加强免疫的组相比，在 外膜囊泡（OMV）加强免疫的组中，腹腔内和浸浴攻击3天后循环中性粒细胞显著增加。在OMV加强免疫的组中，腹腔内和浸浴攻击后，血液中的淋巴细胞分别在7天和14天增加。此外，在OMV加强免疫的组中检测到IgM滴度升高。我们确定一种商业疫苗对 菌株有效，并且OMV是 细胞中最具免疫原性的成分。
