Louisiana State University, Baton Rouge, LA 70803, USA,
Department of Anatomy and Physiology, Kansas State University, Manhattan, KS 66506, USA.
J Zoo Wildl Med. 2024 Jan;54(4):721-727. doi: 10.1638/2022-0151.
A pharmacokinetic study of ceftiofur crystalline free acid sterile oil suspension (CCFA) was performed in six apparently healthy juvenile American alligators (). A single intramuscular dose of 30 mg/kg was administered in the triceps muscle. Blood samples were collected prior to treatment and at 4, 12, 24, 48, 72, 120, 144, 192, 288, and 366 h post administration. Plasma samples were analyzed for ceftiofur equivalent concentrations using liquid chromatography-mass spectrometry. Pharmacokinetic parameters were determined by noncompartmental analysis. Mean peak plasma concentration was 23.2 µg/ml (range, 16.0-27.9), median time to maximum concentration was 72 h (range, 72-120), mean area under the curve from 0 to 366 h postdose was 4.24 h · mg/ml (range, 3.54-4.97), and mean terminal half-life was 143 h (range, 90.8-220). Plasma concentrations were maintained above the minimum inhibitory concentration for this study of 2.0 µg/ml, which was established from similar CCFA pharmacokinetic studies in other reptilian species, through the end of the data collection of 366 h. Because of prolonged plasma concentrations, a dosing interval could not be established in this study. Future studies should include extended collection time points and multidose studies to determine dosing regimens.
在 6 只健康的幼年美洲鳄（）中进行了头孢噻呋游离酸无菌油混悬液（CCFA）的药代动力学研究。在三头肌中肌肉注射 30mg/kg 的单剂量。在治疗前和给药后 4、12、24、48、72、120、144、192、288 和 366 小时采集血样。使用液相色谱-质谱法分析头孢噻呋等效浓度的血浆样品。通过非房室分析确定药代动力学参数。平均峰值血浆浓度为 23.2µg/ml（范围，16.0-27.9），中位数达峰时间为 72 小时（范围，72-120），0 至 366 小时后曲线下面积的平均值为 4.24h·mg/ml（范围，3.54-4.97），平均终末半衰期为 143 小时（范围，90.8-220）。血浆浓度维持在本研究中 2.0µg/ml 的最低抑菌浓度之上，这是从其他爬行动物的类似 CCFA 药代动力学研究中确定的，直至 366 小时数据收集结束。由于血浆浓度延长，本研究无法确定给药间隔。未来的研究应包括延长采集时间点和多剂量研究，以确定给药方案。
