Martinena Camila Belen, Corleto Merlina, Martínez Melina María Belén, Amiano Nicolás Oscar, García Verónica Edith, Maffia Paulo Cesar, Tateosian Nancy Liliana
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina.
Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina.
Cannabis Cannabinoid Res. 2024 Apr;9(2):464-469. doi: 10.1089/can.2023.0124. Epub 2024 Jan 22.
, the etiologic agent of tuberculosis (TB), has killed nearly one billion people during the last two centuries. Nowadays, TB remains a major global health problem ranked among the top 10 causes of death worldwide. One of the main challenges in developing new strategies to fight TB is focused on reducing the duration and complexity of drug regimens. Cannabidiol (CBD) is the main nonpsychoactive ingredient extracted from the . plant, which has been shown to be biologically active against bacteria. The purpose of this work was to investigate the antimicrobial effect of CBD on intracellular infection. To assess the minimum inhibitory concentration (MIC) of CBD on mycobacterial strains, the MTT assay was performed on , and the Colony-Forming Unit (CFU) assay was conducted on H37Rv. Additionally, the cytotoxic effect of CBD on THP-1 cells was assessed by MTT assay. Moreover, macrophages derived from the THP-1 cell were infected with H37Rv (multiplicity of infection 1:10) to evaluate the intracellular activity of CBD by determining the CFU/mL. Antimicrobial activity against (MIC=100 μM) and H37Rv (MIC=25 μM) cultures was exhibited by CBD. Furthermore, the effect of CBD was also evaluated on H37Rv infected macrophage cells. Interestingly, a reduction in viable intracellular H37Rv bacteria was observed after 24 h of treatment. Moreover, CBD exhibited a safe profile toward human THP-1 cells, since it showed no toxicity (CC=1075 μM) at a concentration of antibacterial effect (selectivity index 43). These results extend the knowledge regarding the antimicrobial activity of CBD and demonstrate its ability to kill the human intracellular pathogen
结核分枝杆菌，作为结核病（TB）的病原体，在过去两个世纪里已导致近10亿人死亡。如今，结核病仍然是一个重大的全球健康问题，在全球十大死因中名列前茅。开发抗结核新策略的主要挑战之一集中在缩短药物治疗方案的疗程和降低其复杂性上。大麻二酚（CBD）是从该植物中提取的主要非精神活性成分，已显示出对细菌具有生物活性。这项工作的目的是研究CBD对细胞内感染的抗菌作用。为了评估CBD对分枝杆菌菌株的最低抑菌浓度（MIC），对进行了MTT试验，并对H37Rv进行了菌落形成单位（CFU）试验。此外，通过MTT试验评估了CBD对THP - 1细胞的细胞毒性作用。此外，将源自THP - 1细胞的巨噬细胞用H37Rv感染（感染复数为1:10），通过测定CFU/mL来评估CBD的细胞内活性。CBD对（MIC = 100 μM）和H37Rv（MIC = 25 μM）培养物表现出抗菌活性。此外，还评估了CBD对H37Rv感染的巨噬细胞的作用。有趣的是，治疗24小时后观察到细胞内活的H37Rv细菌数量减少。此外，CBD对人THP - 1细胞表现出安全的特性，因为在抗菌作用浓度下（选择性指数43）它没有显示出毒性（CC = 1075 μM）。这些结果扩展了关于CBD抗菌活性的知识，并证明了其杀死人类细胞内病原体的能力
