鳞翅目夜蛾科的第二个独特α杆状病毒的基因组特征和 ODV 蛋白组。
Genome characteristics and the ODV proteome of a second distinct alphabaculovirus from Spodoptera litura.
机构信息
National Key Laboratory of Agricultural Microbiology, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, 100081, Beijing, China.
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 100101, Beijing, China.
出版信息
BMC Genomics. 2024 Jan 22;25(1):91. doi: 10.1186/s12864-024-09989-3.
BACKGROUND
Spodoptera litura is a harmful pest that feeds on more than 80 species of plants, and can be infected and killed by Spodoptera litura nucleopolyhedrovirus (SpltNPV). SpltNPV-C3 is a type C SpltNPV clone, that was observed and collected in Japan. Compared with type A or type B SpltNPVs, SpltNPV-C3 can cause the rapid mortality of S. litura larvae.
METHODS
In this study, occlusion bodies (OBs) and occlusion-derived viruses (ODVs) of SpltNPV-C3 were purified, and OBs were observed by scanning electron microscopy (SEM). ODVs were observed under a transmission electron microscope (TEM).
RESULTS
Both OBs and ODVs exhibit morphological characteristics typical of nucleopolyhedroviruses (NPVs).The genome of SpltNPV-C3 was sequenced and analyzed; the total length was 148,634 bp (GenBank accession 780,426,which was submitted as SpltNPV-II), with a G + C content of 45%. A total of 149 predicted ORFs were found. A phylogenetic tree of 90 baculoviruses was constructed based on core baculovirus genes. LC‒MS/MS was used to analyze the proteins of SpltNPV-C3; 34 proteins were found in the purified ODVs, 15 of which were core proteins. The structure of the complexes formed by per os infectivity factors 1, 2, 3 and 4 (PIF-1, PIF-2, PIF-3 and PIF-4) was predicted with the help of the AlphaFold multimer tool and predicted conserved sequences in PIF-3. SpltNPV-C3 is a valuable species because of its virulence, and the analysis of its genome and proteins in this research will be beneficial for pest control efforts.
背景
斜纹夜蛾是一种危害超过 80 种植物的有害害虫，可被斜纹夜蛾核型多角体病毒（SpltNPV）感染和杀死。SpltNPV-C3 是一种 C 型 SpltNPV 克隆，在日本被观察和收集。与 A 型或 B 型 SpltNPVs 相比，SpltNPV-C3 可导致斜纹夜蛾幼虫迅速死亡。
方法
本研究中，纯化了 SpltNPV-C3 的多角体和出芽型病毒（ODV），并用扫描电子显微镜（SEM）观察多角体，用透射电子显微镜（TEM）观察 ODV。
结果
多角体和 ODV 均表现出核多角体病毒（NPVs）的典型形态特征。测序和分析了 SpltNPV-C3 的基因组；全长 148634bp（GenBank 登录号为 780426，已作为 SpltNPV-II 提交），G+C 含量为 45%。共发现 149 个预测的 ORFs。基于核心杆状病毒基因构建了 90 种杆状病毒的系统发育树。使用 LC-MS/MS 分析了 SpltNPV-C3 的蛋白质，在纯化的 ODV 中发现了 34 种蛋白质，其中 15 种是核心蛋白。借助 AlphaFold 多聚体工具和 PIF-3 中的预测保守序列，预测了经口感染因子 1、2、3 和 4（PIF-1、PIF-2、PIF-3 和 PIF-4）形成的复合物的结构。由于其毒力，SpltNPV-C3 是一种有价值的物种，本研究对其基因组和蛋白质的分析将有助于害虫防治工作。
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