LIGBCM-AVI, Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes, Bernal, Argentina.
J Virol. 2012 Nov;86(22):12069-79. doi: 10.1128/JVI.01873-12. Epub 2012 Aug 29.
The family Baculoviridae is a large group of insect viruses containing circular double-stranded DNA genomes of 80 to 180 kbp, which have broad biotechnological applications. A key feature to understand and manipulate them is the recognition of orthology. However, the differences in gene contents and evolutionary distances among the known members of this family make it difficult to assign sequence orthology. In this study, the genome sequences of 58 baculoviruses were analyzed, with the aim to detect previously undescribed core genes because of their remote homology. A routine based on Multi PSI-Blast/tBlastN and Multi HaMStR allowed us to detect 31 of 33 accepted core genes and 4 orthologous sequences in the Baculoviridae which were not described previously. Our results show that the ac53, ac78, ac101 (p40), and ac103 (p48) genes have orthologs in all genomes and should be considered core genes. Accordingly, there are 37 orthologous genes in the family Baculoviridae.
杆状病毒科是一组包含 80 到 180 kbp 环状双链 DNA 基因组的昆虫病毒，具有广泛的生物技术应用。理解和操纵它们的一个关键特征是识别同源性。然而，由于该家族已知成员的基因含量和进化距离的差异，使得难以分配序列的同源性。在这项研究中，分析了 58 种杆状病毒的基因组序列，目的是检测由于远程同源性而未被描述的先前核心基因。基于多 PSI-Blast/tBlastN 和多 HaMStR 的常规方法使我们能够检测到杆状病毒科 33 个公认核心基因中的 31 个和 4 个以前未描述的直系同源序列。我们的结果表明，ac53、ac78、ac101（p40）和 ac103（p48）基因在所有基因组中都有同源基因，应被视为核心基因。因此，杆状病毒科有 37 个直系同源基因。
