Liu Zihui, Chen Binglin, Zou Zhiying, Li Dayu, Zhu Jinglin, Yu Jie, Xiao Wei, Yang Hong
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China.
Animals (Basel). 2024 Jan 15;14(2):266. doi: 10.3390/ani14020266.
Hybridization is a widely used breeding technique in fish species that enhances desirable traits in cultured species through heterosis. However, the mechanism by which hybrids alter gene expression to form heterosis remains unclear. In this study, a group of hybrid tilapia was used to elucidate heterosis through interspecies crossing. Specifically, was analyzed to describe the regulation of gene expression variation in hybrid tilapia. Transcripts from the Nile tilapia allele were found to be significantly higher than those from the blue tilapia allele in hybrid individuals, indicating that the expression of was dominated by Nile tilapia sub-genomic alleles. The study also found a compensatory interaction of cis- and trans-acting elements of the Nile tilapia and blue tilapia sub-genomes, inducing a non-additive expression of in hybrids. Eight specific SNPs were identified in the promoter regions of Nile tilapia and blue tilapia, and were found to be promoter differences leading to differences in gene expression efficiencies between parental alleles using a dual-luciferase reporter system. This study provides insights into the non-additive expression patterns of key functional genes in fish hybrids related to growth and immunity response.
杂交是一种在鱼类中广泛应用的育种技术，它通过杂种优势增强养殖品种的优良性状。然而，杂种改变基因表达以形成杂种优势的机制仍不清楚。在本研究中，一组杂交罗非鱼被用于通过种间杂交阐明杂种优势。具体而言，对其进行分析以描述杂交罗非鱼基因表达变异的调控。在杂交个体中，尼罗罗非鱼等位基因的转录本显著高于蓝罗非鱼等位基因的转录本，这表明[此处原文缺失基因名称]的表达由尼罗罗非鱼亚基因组等位基因主导。该研究还发现尼罗罗非鱼和蓝罗非鱼亚基因组顺式和反式作用元件之间的补偿性相互作用，诱导了杂种中[此处原文缺失基因名称]的非加性表达。在尼罗罗非鱼和蓝罗非鱼的[此处原文缺失基因名称]启动子区域鉴定出八个特定的单核苷酸多态性（SNP），并且使用双荧光素酶报告系统发现这些是导致亲本等位基因之间基因表达效率差异的启动子差异。本研究为鱼类杂种中与生长和免疫反应相关的关键功能基因的非加性表达模式提供了见解。
