Cho Young-Eun, Chen Shaoshuai, Crouch Keith, Yun Joseph, Klingelhutz Aloysius
College of Nursing, The University of Iowa, 50 Newton Road, Iowa City, IA 52242, USA.
Predictiv Care, Inc., 800 West El Camino Real, Mountain View, CA 94040, USA.
Biomedicines. 2024 Jan 3;12(1):100. doi: 10.3390/biomedicines12010100.
Middle-aged adults have the highest obesity rates, leading to significant health complications in later years. Obesity triggers the release of altered molecules, including extracellular vesicles (EVs) from excess adipose tissue (AT), contributing to various health complications. In this study, we assessed the effects of age and a high-fat diet on AT-derived EV miRNA profiles to understand their potential roles in aging and obesity.
C57BL/6 male mice were subjected to a normal chow diet (NCD) or a high-fat diet (HFD) for either 10-12 weeks (young mice, = 10) or 50-61 weeks (middle-aged mice, = 12). After evaluating metabolic characteristics, peri-gonadal white AT was isolated and cultured to obtain EVs. AT-derived EV miRNAs were profiled using a NanoString miRNA panel ( = 599).
Middle-aged mice exhibited obesity regardless of diet. Young mice fed an HFD showed similar metabolic traits to middle-aged mice. In the NCD group, 131 differentially expressed miRNAs (DE-miRNAs) emerged in middle-aged mice compared to young mice, including miR-21, miR-148a, and miR-29a, associated with cancer, neuro/psychological disorders, and reproductive diseases. In the HFD group, 55 DE-miRNAs were revealed in middle-aged mice compared to young mice. These miRNAs were associated with significantly suppressed IGF1R activity.
This study demonstrates the potential significant impact of miRNAs of AT EVs on aging- and obesity-related diseases.
中年成年人肥胖率最高，这会在晚年引发严重的健康并发症。肥胖会触发包括来自多余脂肪组织（AT）的细胞外囊泡（EVs）在内的分子变化，从而导致各种健康并发症。在本研究中，我们评估了年龄和高脂饮食对AT来源的EV miRNA谱的影响，以了解它们在衰老和肥胖中的潜在作用。
将C57BL/6雄性小鼠分为正常饮食组（NCD）或高脂饮食组（HFD），喂养10 - 12周（年轻小鼠，n = 10）或50 - 61周（中年小鼠，n = 12）。在评估代谢特征后，分离并培养性腺周围白色脂肪组织以获得EVs。使用NanoString miRNA芯片（n = 599）对AT来源的EV miRNAs进行分析。
无论饮食如何，中年小鼠均出现肥胖。喂食HFD的年轻小鼠表现出与中年小鼠相似的代谢特征。在NCD组中，与年轻小鼠相比，中年小鼠出现了131种差异表达的miRNA（DE - miRNAs），包括与癌症、神经/心理障碍和生殖疾病相关的miR - 21、miR - 148a和miR - 29a。在HFD组中，与年轻小鼠相比，中年小鼠发现了55种DE - miRNAs。这些miRNA与显著抑制的IGF1R活性相关。
本研究证明了AT EVs的miRNAs对衰老和肥胖相关疾病具有潜在的重大影响。
