Key Laboratory of East China Sea Fishery Resources Exploitation, Ministry of Agriculture and Rural Affairs, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China.
Centre for Research on Environmental Ecology and Fish Nutrition (CREEFFN) of the Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China.
Int J Mol Sci. 2024 Jan 5;25(2):700. doi: 10.3390/ijms25020700.
Variability in food availability leads to condition-dependent investments in reproduction. This study is aimed at understanding the metabolic response and regulatory mechanism of female in response to starvation in a temporal- and tissue-specific manner. The mud crabs were starved for 7 (control), 14, 28, and 40 days for histological and biochemical analysis in the hepatopancreas, ovary, and serum, as well as for RNA sequencing on the hepatopancreas and ovary. We further highlighted candidate gene modules highly linked to physiological traits. Collectively, our observations suggested that starvation triggered endogenous ovarian maturation at the expense of hepatopancreas mass, with both metabolic adjustments to optimize energy and fatty acid supply from hepatopancreas to ovary in the early phase, followed by the activation of autophagy-related pathways in both organs over prolonged starvation. These specific adaptive responses might be considered efficient strategies to stimulate ovarian maturation of under fasting stress, which improves the nutritional value of female mud crabs and other economically important crustaceans.
食物可获得性的变化导致了繁殖的条件依赖性投资。本研究旨在从时间和组织特异性的角度，了解雌性在饥饿状态下的代谢反应和调控机制。将泥蟹饥饿 7（对照）、14、28 和 40 天，用于肝胰腺、卵巢和血清的组织学和生化分析，以及肝胰腺和卵巢的 RNA 测序。我们进一步强调了与生理特征高度相关的候选基因模块。总的来说，我们的观察表明，饥饿以牺牲肝胰腺质量为代价触发了内源卵巢成熟，在早期阶段，代谢进行了调整，以优化从肝胰腺向卵巢提供能量和脂肪酸，随后在长时间饥饿的情况下，两个器官中的自噬相关途径被激活。这些特定的适应性反应可能被认为是在禁食应激下刺激雌性卵巢成熟的有效策略，这提高了雌性泥蟹和其他经济上重要的甲壳类动物的营养价值。
