Słowiński Krzysztof, Grygierzec Beata, Baran Agnieszka, Tabor Sylwester, Piatti Diletta, Maggi Filippo, Synowiec Agnieszka
Department of Forest Management, Engineering and Technology, University of Agriculture in Krakow, al. 29 Listopada 46, 31-425 Krakow, Poland.
Department of Agroecology and Crop Production, University of Agriculture in Krakow, al. Mickiewicza 21, 31-120 Krakow, Poland.
Plants (Basel). 2024 Jan 5;13(2):152. doi: 10.3390/plants13020152.
Japanese knotweed ( Houtt.) is Poland's invasive weed, for which there is no efficient control method. The rhizomes of this species are rich in resveratrol. In this work, we evaluated (1) the effectiveness of electromagnetic microwaves (MV) in destroying Japanese knotweed using an original device, HOGWEED (MV of 2450 MHz), (2) the ecotoxic effect of the MV on the soil environment, and (3) the resveratrol content in knotweed rhizomes after MV treatment. The field studies were carried out in 2022 in southern Poland. Cut plants were MV-treated for times of 5.0-25.0 min. The MV efficiency was checked 10 and 56 days after treatment (DAT). After MV treatment, fresh soil samples were taken to analyze their ecotoxicity. As a result, at 56 DAT, knotweed was controlled if MV was used for at least 20.0 min. The MV did not affect the soil ecotoxicity. The MV-treated soils were classified as non-toxic or low-toxic. To analyze the resveratrol content, healthy knotweed rhizomes were dug out, treated with MV in the laboratory at 2.5-10.0 min, and analyzed for resveratrol content in HPLC-MS/MS. As a result, the resveratrol in the rhizomes significantly decreased in a time-dependent manner following MV exposure.
虎杖（Houtt.）是波兰的一种入侵性杂草，目前尚无有效的防治方法。该物种的根茎富含白藜芦醇。在本研究中，我们评估了：（1）使用一种名为HOGWEED（2450 MHz微波）的原创设备，电磁微波（MV）对虎杖的杀灭效果；（2）MV对土壤环境的生态毒性影响；（3）MV处理后虎杖根茎中的白藜芦醇含量。田间试验于2022年在波兰南部进行。将砍下的植株用MV处理5.0 - 25.0分钟。在处理后10天和56天检查MV的效果。MV处理后，采集新鲜土壤样本分析其生态毒性。结果表明，在处理后56天，如果使用MV至少20.0分钟，虎杖就能得到控制。MV对土壤生态毒性没有影响。经MV处理的土壤被归类为无毒或低毒。为了分析白藜芦醇含量，挖出健康的虎杖根茎，在实验室用MV处理2.5 - 10.0分钟，然后用高效液相色谱 - 串联质谱法分析根茎中的白藜芦醇含量。结果显示，MV处理后，根茎中的白藜芦醇含量随时间呈显著下降趋势。
