Guo Beiyi, Chen Hong, Yin Yuying, Wang Wei, Zeng Songjun
Guangzhou Collaborative Innovation Center on Science-Tech of Ecology and Landscape, Guangzhou Landscape Plant Germplasm Resource Nursery, Guangzhou Institute of Forestry and Landscape Architecture, Guangzhou 510540, China.
Key Laboratory of South China Agricultural Plant Molecular Analysis and Gene Improvement, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
Plants (Basel). 2024 Jan 11;13(2):197. doi: 10.3390/plants13020197.
This study successfully established an efficient in vitro propagation system for SCBG Huihuang90 via protocorm-like body (PLB) formation from seed-derived calluses, PLB proliferation and differentiation, root induction and greenhouse acclimatization. Furthermore, 1/2 Murashige and Skoog (MS) + 0.025 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D) was suitable for the proliferation of PLBs, and 1/2MS + 10% coconut water (CW, /) + 0.5 g/L activated carbon (AC) was suitable for PLB differentiation. PLBs at different developmental stages required different kinds of sugars. This study provided a reference for research on the propagation techniques of other .
本研究通过从种子愈伤组织形成类原球茎（PLB）、PLB增殖与分化、生根诱导及温室驯化，成功建立了高效的蕙兰‘辉煌90’离体繁殖体系。此外，1/2 Murashige和Skoog（MS）+ 0.025 mg/L 2,4-二氯苯氧乙酸（2,4-D）适合PLB增殖，1/2MS + 10%椰汁（CW）+ 0.5 g/L活性炭（AC）适合PLB分化。不同发育阶段的PLB需要不同种类的糖。本研究为其他[植物名称缺失]繁殖技术的研究提供了参考。
