Liang Yuqing, Li Xiaoshuang, Lei Feiya, Yang Ruirui, Bai Wenwan, Yang Qilin, Zhang Daoyuan
State Key Laboratory of Desert and Oasis Ecology, Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.
Turpan Eremophytes Botanical Garden, Chinese Academy of Sciences, Turpan 838008, China.
Plants (Basel). 2024 Jan 11;13(2):205. doi: 10.3390/plants13020205.
Desiccation is a kind of extreme form of drought stress and desiccation tolerance (DT) is an ancient trait of plants that allows them to survive tissue water potentials reaching -100 MPa or lower. is a DREB A-5 transcription factor gene from a DT moss named , which has strong comprehensive tolerance to osmotic and salt stresses. This study delves further into the molecular mechanism of ScDREB10 stress tolerance based on the transcriptome data of the overexpression of in under control, osmotic and salt treatments. The transcriptional analysis of weight gene co-expression network analysis (WGCNA) showed that "phenylpropanoid biosynthesis" and "starch and sucrose metabolism" were key pathways in the network of cyan and yellow modules. Meanwhile, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of differentially expressed genes (DEGs) also showed that "phenylpropanoid biosynthesis" and "starch and sucrose metabolism" pathways demonstrate the highest enrichment in response to osmotic and salt stress, respectively. Quantitative real-time PCR (qRT-PCR) results confirmed that most genes related to phenylpropanoid biosynthesis" and "starch and sucrose metabolism" pathways in overexpressing were up-regulated in response to osmotic and salt stresses, respectively. In line with the results, the corresponding lignin, sucrose, and trehalose contents and sucrose phosphate synthase activities were also increased in overexpressing ScDREB10 under osmotic and salt stress treatments. Additionally, cis-acting promoter element analyses and yeast one-hybrid experiments showed that ScDREB10 was not only able to bind with classical cis-elements, such as DRE and TATCCC (MYBST1), but also bind with unknown element CGTCCA. All of these findings suggest that ScDREB10 may regulate plant stress tolerance by effecting phenylpropanoid biosynthesis, and starch and sucrose metabolism pathways. This research provides insights into the molecular mechanisms underpinning ScDREB10-mediated stress tolerance and contributes to deeply understanding the A-5 DREB regulatory mechanism.
脱水是干旱胁迫的一种极端形式，而耐脱水性（DT）是植物的一种古老特性，使它们能够在组织水势达到-100 MPa或更低时存活。ScDREB10是来自一种名为的耐脱水苔藓的DREB A-5转录因子基因，对渗透胁迫和盐胁迫具有很强的综合耐受性。本研究基于在对照、渗透和盐处理下ScDREB10过表达的转录组数据，进一步深入探究其胁迫耐受性的分子机制。加权基因共表达网络分析（WGCNA）的转录分析表明，“苯丙烷生物合成”和“淀粉与蔗糖代谢”是青色和黄色模块网络中的关键途径。同时，对差异表达基因（DEG）的基因本体（GO）和京都基因与基因组百科全书（KEGG）分析也表明，“苯丙烷生物合成”和“淀粉与蔗糖代谢”途径分别在响应渗透胁迫和盐胁迫时表现出最高的富集度。定量实时PCR（qRT-PCR）结果证实，在过表达ScDREB10的情况下，与“苯丙烷生物合成”和“淀粉与蔗糖代谢”途径相关的大多数基因分别在响应渗透胁迫和盐胁迫时上调。与结果一致，在渗透和盐胁迫处理下过表达ScDREB10时，相应的木质素、蔗糖和海藻糖含量以及蔗糖磷酸合酶活性也增加。此外，顺式作用启动子元件分析和酵母单杂交实验表明，ScDREB10不仅能够与经典的顺式元件如DRE和TATCCC（MYBST1）结合，还能与未知元件CGTCCA结合。所有这些发现表明，ScDREB10可能通过影响苯丙烷生物合成以及淀粉和蔗糖代谢途径来调节植物的胁迫耐受性。本研究为深入了解ScDREB10介导的胁迫耐受性的分子机制提供了见解，并有助于深入理解A-5 DREB调控机制。
