CAS Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Xinjiang Urumqi 830011, China.
University of Chinese Academy of Sciences, Beijing 100049, China.
Genes (Basel). 2019 Feb 14;10(2):146. doi: 10.3390/genes10020146.
Drought and salinity are major factors limiting crop productivity worldwide. DREB (dehydration-responsive element-binding) transcription factors play important roles in plant stress response and have been identified in a wide variety of plants. Studies on DREB are focused on the A-1 () and A-2 () groups. Studies on A-5 group DREBs, which represent a large proportion of the DREB subfamily, is limited. In this study, we characterized and analyzed the stress tolerance function of an A-5c type gene from the desert moss . Transactivation assay in yeast showed that ScDREB10 had transactivation activity. Transient expression assay revealed that ScDREB10 was distributed both in the nucleus and cytosol of tobacco leaf epidermal cells. Overexpression of significantly increased the germination percentage of seeds under osmotic and salt stresses, and improved the osmotic and salt stress tolerances of at the seedling stage and is associated with the expression of downstream stress-related genes and improved reactive oxygen species (ROS) scavenging ability. Our study provides insight into the molecular mechanism of stress tolerance of A-5 type DREB proteins, as well as providing a promising candidate gene for crop salt and drought stress breeding.
干旱和盐度是全球范围内限制作物生产力的主要因素。DREB（脱水响应元件结合）转录因子在植物应激反应中发挥重要作用，已在多种植物中被鉴定出来。对 DREB 的研究主要集中在 A-1（）和 A-2（）两组。对 A-5 组 DREBs 的研究，占 DREB 亚家族的很大一部分，是有限的。在这项研究中，我们对来自沙漠苔藓的一种 A-5c 型基因进行了特征描述和分析。酵母中的转激活测定表明 ScDREB10 具有转激活活性。瞬时表达分析表明 ScDREB10 分布在烟草叶片表皮细胞的细胞核和细胞质中。过表达 显著增加了在渗透和盐胁迫下种子的发芽率，并提高了在幼苗期对渗透和盐胁迫的耐受性，这与下游应激相关基因的表达和提高活性氧（ROS）清除能力有关。我们的研究为 A-5 型 DREB 蛋白的应激耐受分子机制提供了深入的了解，并为作物的盐和干旱胁迫育种提供了有前途的候选基因。
