Rajkonwar Jadab, Shende Varun, Maji Ananta Kumar, Pandey Apoorva, Sharma Puran K, Gunasekaran Kasinathan, Subbarao Sarala K, Bhattacharyya Dibya Ranjan, Raghavendra Kamaraju, Pebam Rocky, Mayakrishnan Vijay, Gogoi Phiroz, Senapati Susmita, Sarkar Pallabi, Biswas Saurav, Debbarma Daniel, Nirmolia Tulika, Jena Sasmita Rani, Bayan Bahniman, Talukder Pinki, Sihag Ashwarya Kumari, Bharali Himadri Sankar, Verma Anisha, Mahanta Kongkon, Sumer Gonsalo, Karmakar Ranjan, Patgiri Saurav Jyoti, Chaudhuri Supriya, Ganguli Sumit, Kaur Harpreet, Bhattacharyya Tapas K, Joshi Pyare Laal, Goswami Bidhan, Baruah Kalpana, Pati Sanghamitra, Narain Kanwar, Bhowmick Ipsita Pal
Regional Medical Research Centre, Northeast Region (RMRC-NE)-ICMR, Dibrugarh 786001, India.
District Health & Family Welfare Samiti, Alipurduar 736121, India.
Microorganisms. 2024 Jan 3;12(1):95. doi: 10.3390/microorganisms12010095.
A comprehensive entomological survey was undertaken in Alipurduar District, West Bengal, from 2018 to 2020 and in 2022. This study was prompted by reported malaria cases and conducted across nine villages, seven Sub-Centres, and three Primary Health Centres (PHCs). Mosquitoes were hand-collected with aspirators and flashlights from human dwellings and cattle sheds during the daytime. Both morphological and molecular techniques were used for species identification. Additionally, mosquitoes were tested for parasites and human blood presence. Mosquito species such as , , , and were morphologically identified. For species like , , , and ., a combination of morphological and molecular techniques was essential. The mitochondrial cytochrome c oxidase gene subunit 1 (CO1) was sequenced for , , , , and some damaged samples, revealing the presence of and . The major species were ., , and ., especially in Kumargram and Turturi PHCs. positivity was notably high in and with significant human blood meal positivity across most species. Morphological, molecular, and phylogenetic analyses are crucial, especially for archived samples, to accurately identify the mosquito fauna of a region. Notably, this study confirms the first occurrence of and in West Bengal and implicates ., , and as significant vectors in the Alipurduar region.
2018年至2020年以及2022年期间，在西孟加拉邦的阿里布尔杜阿尔区开展了一项全面的昆虫学调查。这项研究是由报告的疟疾病例引发的，调查覆盖了9个村庄、7个分中心和3个初级卫生保健中心（PHC）。白天，研究人员使用吸虫器和手电筒从人类住所和牛棚中手工收集蚊子。形态学和分子技术都被用于物种鉴定。此外，还对蚊子进行了寄生虫和人类血液检测。诸如 、 、 和 等蚊种通过形态学进行了鉴定。对于 、 、 和 等蚊种，形态学和分子技术相结合是必不可少的。对 、 、 、 以及一些受损样本的线粒体细胞色素c氧化酶基因亚基1（CO1）进行了测序，结果显示存在 和 。主要的 蚊种为 、 和 ，特别是在库马尔格拉姆和图尔图里初级卫生保健中心。 在 和 中的阳性率显著较高，且大多数蚊种的人类血餐阳性率也很高。形态学、分子学和系统发育分析至关重要，特别是对于存档样本，以准确识别一个地区的蚊虫种类。值得注意的是，这项研究证实了 和 在西孟加拉邦首次出现，并表明 、 和 是阿里布尔杜阿尔地区的重要病媒。
