Mohanty Amitav, Kar Priyanka, Mishra Kirti, Singh Durg V, Mohapatra Namita, Kar Santanu K, Dash Aditya P, Hazra Rupenangshu K
Division of Vector Borne Diseases, Institute of Life Sciences, Bhubaneswar, India.
Am J Trop Med Hyg. 2007 May;76(5):837-43.
A multiplex PCR assay has been developed for detection of Anopheles fluviatilis cryptic species, their human host preference, and Plasmodium falciparum presence in the mosquito. PCR conditions were optimized using primer sets specific for A. fluviatilis cryptic species, Homo sapiens, and P. falciparum and evaluated with field-collected mosquitoes. A unique mosquito processing method was used for screening P. falciparum carrying capacity and human host preference of A. fluviatilis mosquitoes in first-round multiplex PCR. The vectorial status of the mosquito for P. falciparum parasite was confirmed in second-round PCR. Of the 121 collected mosquitoes, 92 were of S type, 26 of T type, and 3 were of other types. Human host preference was dominant in S type, of which 4% were P. falciparum sporozoite positive. This assay and processing method can also be used to evaluate vector competence of other anophelines.
已开发出一种多重PCR检测方法，用于检测溪流按蚊隐种、它们对人类宿主的偏好以及疟原虫在蚊子体内的存在情况。使用针对溪流按蚊隐种、智人和恶性疟原虫的引物对优化PCR条件，并对野外采集的蚊子进行评估。在第一轮多重PCR中，采用一种独特的蚊子处理方法来筛选溪流按蚊携带恶性疟原虫的能力及其对人类宿主的偏好。在第二轮PCR中确认了该蚊子作为恶性疟原虫寄生虫媒介的状态。在收集的121只蚊子中，92只为S型，26只为T型，3只为其他类型。S型中对人类宿主的偏好占主导，其中4%的蚊子恶性疟原虫子孢子呈阳性。该检测方法和处理方法也可用于评估其他按蚊的媒介能力。
